Two white collar proteins protect fungal cells from solar UV damage by their interactions with two photolyases in Metarhizium robertsii
Open Access
- 31 August 2021
- journal article
- research article
- Published by Wiley in Environmental Microbiology
- Vol. 23 (9), 4925-4938
- https://doi.org/10.1111/1462-2920.15398
Abstract
The photolyases PHR1 and PHR2 enable photorepair of fungal DNA lesions in the forms of UV-induced cyclobutane pyrimidine dimer (CPD) and (6-4)-pyrimidine-pyrimidone (6-4PP) photoproducts, but their regulation remains mechanistically elusive. Here, we report that the white collar proteins WC1 and WC2 mutually interacting to form a light-responsive transcription factor regulate photolyase expression required for fungal UV resistance in the insect-pathogenic fungus Metharhizum robertsii. Conidial UVB resistance decreased by 54% in Delta wc1 and 67% in Delta wc2. Five-hour exposure of UVB-inactivated conidia to visible light resulted in photoreactivation rates of 30% and 9% for the Delta wc1 and Delta wc2 mutants, contrasting to 79%-82% for wild-type and complemented strains. Importantly, abolished transcription of phr1 in Delta wc-2 and of phr2 in Delta wc1 resulted in incapable photorepair of CDP and 6-4PP DNA lesions in UVB-impaired Delta wc2 and Delta wc1 cells respectively. Yeast two-hybrid assays revealed interactions of either WC protein with both PHR1 and PHR2. Therefore, the essential roles for WC1 and WC2 in both photorepair of UVB-induced DNA lesions and photoreactivation of UVB-inactivated conidia rely upon their interactions with, and hence transcriptional activation of, PHR1 and PHR2. These findings uncover a novel WC-cored pathway that mediates filamentous fungal response and adaptation to solar UV irradiation.Funding Information
- Ministry of Science and Technology of the People's Republic of China (2017YFD0201202)
- National Natural Science Foundation of China (31772218, 31801795)
This publication has 51 references indexed in Scilit:
- Enhanced UV Resistance and Improved Killing of Malaria Mosquitoes by Photolyase Transgenic Entomopathogenic FungiPLOS ONE, 2012
- The circadian clock ofNeurospora crassaFEMS Microbiology Reviews, 2012
- The Cryptochromes: Blue Light Photoreceptors in Plants and AnimalsAnnual Review of Plant Biology, 2011
- Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridumPLoS Genetics, 2011
- In vitro and in vivo responses of fungal biocontrol agents to gradient doses of UV-B and UV-A irradiationBioControl, 2010
- Trichoderma atroviride PHR1, a Fungal Photolyase Responsible for DNA Repair, Autoregulates Its Own PhotoinductionEukaryotic Cell, 2007
- Rad4–Rad23 interaction with SWI/SNF links ATP-dependent chromatin remodeling with nucleotide excision repairNature Structural & Molecular Biology, 2006
- Light Controls Growth and Development via a Conserved Pathway in the Fungal KingdomPLoS Biology, 2005
- Effects of simulated solar radiation on conidial germination of Metarhizium anisopliae in different formulationsCrop Protection, 1998
- Site-specific DNA repair at the nucleosome level in a yeast minichromosomeCell, 1990