Consolidated bioprocessing performance of a two-species microbial consortium for butanol production from lignocellulosic biomass
- 1 October 2020
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 117 (10), 2985-2995
- https://doi.org/10.1002/bit.27464
Abstract
Consolidated bioprocessing (CBP) by using microbial consortium was considered as a promising approach to achieve direct biofuel production from lignocellulose. In this study, the interaction mechanism of microbial consortium consisting ofThermoanaerobacterium thermosaccharolyticumM5 andClostridium acetobutylicumNJ4 was analyzed, which could achieve efficient butanol production from xylan through CBP. Strain M5 possesses efficient xylan degradation capability, as 19.73 g/L of xylose was accumulated within 50 hr. The efficient xylose utilization capability of partner strain NJ4 could relieve the substrate inhibition to hydrolytic enzymes of xylanase and xylosidase secreted by strain M5. In addition, the earlier solventogenesis of strain NJ4 was observed due to the existence of butyrate generated by strain M5. The mutual interaction of these two strains finally gave 13.28 g/L of butanol from 70 g/L of xylan after process optimization, representing a relatively high butanol production from hemicellulose. Moreover, 7.61 g/L of butanol was generated from untreated corncob via CBP. This successfully constructed microbial consortium exhibits efficient cooperation performance on butanol production from lignocellulose, which could provide a platform for the emerging butanol production from lignocellulose.Funding Information
- National Natural Science Foundation of China (21706125, 21978130, 31961133017)
- State Key Laboratory of Materials- Oriented Chemical Engineering (ZK201601)
This publication has 28 references indexed in Scilit:
- Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysisBiotechnology for Biofuels, 2019
- Design and construction of synthetic microbial consortia in ChinaSynthetic and Systems Biotechnology, 2016
- Butanol production from lignocellulose by simultaneous fermentation, saccharification, and pervaporation or vacuum evaporationBioresource Technology, 2016
- Metabolic engineering of Clostridium cellulolyticum for the production of n-butanol from crystalline celluloseMicrobial Cell Factories, 2016
- Butanol production from acid hydrolyzed corn fiber with Clostridium beijerinckii mutantBioresource Technology, 2012
- High yield bio-butanol production by solvent-producing bacterial microfloraBioresource Technology, 2012
- Natural strategies for the spatial optimization of metabolism in synthetic biologyNature Chemical Biology, 2012
- Progress in the production and application of n-butanol as a biofuelRenewable and Sustainable Energy Reviews, 2011
- Economical challenges to microbial producers of butanol: Feedstock, butanol ratio and titerBiotechnology Journal, 2011
- Improvement of xylose utilization in Clostridium acetobutylicum via expression of the talA gene encoding transaldolase from Escherichia coliJournal of Biotechnology, 2009