PLGA-collagen-ECM hybrid meshes mimicking stepwise osteogenesis and their influence on the osteogenic differentiation of hMSCs
- 20 February 2020
- journal article
- research article
- Published by IOP Publishing in Biofabrication
- Vol. 12 (2), 025027
- https://doi.org/10.1088/1758-5090/ab782b
Abstract
Extracellular matrixes (ECMs) are dynamically altered and remodeled during tissue development. How the dynamic remodeling of ECM affects stem cell functions remains poorly understood due to the difficulty of obtaining biomimetic ECMs. In this study, stepwise osteogenesis-mimicking ECM-deposited hybrid meshes were prepared by culturing human mesenchymal stem cells (hMSCs) in poly (lactic-coglycolic acid) (PLGA)-collagen hybrid meshes and controlling the stages of the osteogenesis of hMSCs. Three types of hybrid mesh mimicking the ECMs from stem cell stage of hMSCs (SC-ECM), early stage (EO-ECM) and late stage (LO-ECM) osteogenesis of hMSCs were prepared. The stepwise osteogenesis-mimicking ECM deposited PLGA-collagen hybrid meshes showed different ECM compositions associated with the stage of osteogenesis, their effects on the osteogenic differentiation of hMSCs differed. EO-ECM scaffold increased and LO-ECM scaffold moderately promoted the osteogenic differentiation of hMSCs. However, SC-ECM scaffold inhibited the osteogenic differentiation of hMSCs. The novel PLGA-collagen-ECM hybrid meshes will provide useful tools for stem cell culture and tissue engineering.Keywords
Funding Information
- JSPS KAKENHI (Grant Numbers 18K19947, 18K19945 and 19H04475)
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