Novel technology for storage and distribution of live vaccines and other biological medicines at ambient temperature
Open Access
- 1 March 2020
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 6 (10), eaau4819
- https://doi.org/10.1126/sciadv.aau4819
Abstract
A novel, thin-film platform that preserves live viruses, bacteria, antibodies, and enzymes without refrigeration for extended periods of time is described. Studies with recombinant adenovirus in an optimized formulation that supports recovery of live virus through 16 freeze-thaw cycles revealed that production of an amorphous solid with a glass transition above room temperature and nitrogen-hydrogen bonding between virus and film components are critical determinants of stability. Administration of live influenza virus in the optimized film by the sublingual and buccal routes induced antibody-mediated immune responses as good as or better than those achieved by intramuscular injection. This work introduces the possibility of improving global access to a variety of medicines by offering a technology capable of reducing costs of production, distribution, and supply chain maintenance.Funding Information
- National Institute of Allergy and Infectious Diseases (U01 AI 078045)
This publication has 56 references indexed in Scilit:
- The impact of making vaccines thermostable in Niger's vaccine supply chainVaccine, 2012
- The Burden of Disease and the Changing Task of MedicineNew England Journal of Medicine, 2012
- Water Soluble Polymers for Pharmaceutical ApplicationsPolymers, 2011
- Cellular immune response in the presence of protective antibody levels correlates with protection against 1918 influenza in ferretsVaccine, 2011
- Development of a nasal adenovirus-based vaccine: Effect of concentration and formulation on adenovirus stability and infectious titer during actuation from two delivery devicesVaccine, 2010
- Thermal Stability of Adenovirus type 2 as a Function of pHJournal of Pharmaceutical Sciences, 2006
- Utility of PEGylated recombinant adeno-associated viruses for gene transferJournal of Controlled Release, 2005
- Development of stable liquid formulations for adenovirus-based vaccinesJournal of Pharmaceutical Sciences, 2004
- Development of formulations that enhance physical stability of viral vectors for gene therapyGene Therapy, 2001
- Development of a Rapid Method for the PEGylation of Adenoviruses with Enhanced Transduction and Improved Stability under Harsh Storage ConditionsHuman Gene Therapy, 2000