Immunophenotyping Investigation in Acute and Chronic Leukemias as a Gold Standard Test

Abstract

Immunophenotyping of leukemias and other hematological neoplasms has become one of the most relevant clinical applications of flow cytometry which remains an indispensable tool for the diagnosis, classification, staging as well as hematologic malignancies monitoring. It can provide quantitative data on a number of cell parameters for a large number of cells in the region of cells thousands per second which making it a powerful diagnostic tool. If immunophenotyping is more sensitive significantly, specific and objective than morphological studying, the latter remains the gold standard criterion for creating and establishing the blast percentage in presentation and post treatment together. Moreover, it is important that interpreters of flow cytometric data have the phenotypes exact knowledge of diverse normal cell populations, which can be one of the best tool in recognize deviations from normal as well as are able to discuss the potential clinical significance and knowing look of the flow cytometric findings.