Genotyping of enteroviruses and human parechoviruses highlights their diversity in Northwest Ethiopia
- 1 December 2020
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 92 (12), 3007-3015
- https://doi.org/10.1002/jmv.25765
Abstract
Enteroviruses (EVs) and human parechoviruses (HPeVs) infections are associated with various forms of disease, including gastroenteritis. As information on the molecular epidemiology of these viruses is limited in Ethiopia, the genetic diversity of EV and HPeV was investigated in the Northwestern part of the country. Of the total 450 stool samples obtained from infants and young children with diarrhea, 157 (34.9%) were positive for EV and 49 (10.9%) for HPeV RNA when tested by real-time reverse transcription polymerase chain reaction. Genotyping was performed by sequencing of the EV VP1 gene and the HPeV VP3/VP1 gene, respectively. Genotyping of EV was successful in 118 samples. Thereof, 82 (69.5%) belonged to non-polio EVs as a broad range of genotypes within species C, B, and A. Sabin polioviruses were found in 36 cases. HPeV sequences were also heterogeneous with a relative dominance of genotype 3. In conclusion, diverse EV and HPeV genotypes were found cocirculating in Northwest Ethiopia. The findings highlight the importance of continuous surveillance of these viruses in Ethiopia.This publication has 23 references indexed in Scilit:
- Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR AssayAdvances in Virology, 2016
- Genotyping of enteroviruses isolated in Kenya from pediatric patients using partial VP1 regionSpringerPlus, 2016
- Detection and characterization of enteroviruses and parechoviruses in healthy people living in the South of Côte d’IvoireJournal of Clinical Virology, 2015
- Molecular analysis of enterovirus in Cameroon by partial 5′UTR-VP4 gene sequencing reveals a high genetic diversity and frequency of infectionsJournal of Medical Virology, 2014
- Picornavirus and enterovirus diversity with associated human diseasesInfection, Genetics and Evolution, 2013
- Detection of human enterovirus and human parechovirus (HPeV) genotypes from clinical stool samples: polymerase chain reaction and direct molecular typing, culture characteristics, and serotypingDiagnostic Microbiology and Infectious Disease, 2010
- Vaccine-derived poliovirus (VDPV): Impact on poliomyelitis eradicationVaccine, 2009
- A convenient rapid culture assay for the detection of enteroviruses in clinical samples: comparison with conventional cell culture and RT-PCRJournal of Medical Microbiology, 2008
- Sensitive, Seminested PCR Amplification of VP1 Sequences for Direct Identification of All Enterovirus Serotypes from Original Clinical SpecimensJournal of Clinical Microbiology, 2006
- VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATIONAnnual Review of Microbiology, 2005