Since the 20th century, antibiotic resistance has been regarded as one of the important factors af-fecting human health, and has posed a serious threat to global economic development. Therefore, the development and application of new antibiotics is urgent. Natural pyrrolomycinsare polyhalo-genated metabolites isolated from the fermentation broth of Actinomyces and Streptomyces. Be-cause of its significant antibacterial activity and novel chemical structure, it has great potential to become a new type of antibacterial agent, and has received extensive attention in the field of medi-cine and pesticides. The research progress on the isolation, biosynthesis, total synthesis and biolog-ical activity of natural pyrrolomycins was reviewed.