Connected function of PRAF/RLD and GNOM in membrane trafficking controls intrinsic cell polarity in plants
Open Access
- 10 January 2022
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 13 (1), 1-17
- https://doi.org/10.1038/s41467-021-27748-w
Abstract
Cell polarity is a fundamental feature underlying cell morphogenesis and organismal development. In the Arabidopsis stomatal lineage, the polarity protein BASL controls stomatal asymmetric cell division. However, the cellular machinery by which this intrinsic polarity site is established remains unknown. Here, we identify the PRAF/RLD proteins as BASL physical partners and mutating four PRAF members leads to defects in BASL polarization. Members of PRAF proteins are polarized in stomatal lineage cells in a BASL-dependent manner. Developmental defects of the praf mutants phenocopy those of the gnom mutants. GNOM is an activator of the conserved Arf GTPases and plays important roles in membrane trafficking. We further find PRAF physically interacts with GNOM in vitro and in vivo. Thus, we propose that the positive feedback of BASL and PRAF at the plasma membrane and the connected function of PRAF and GNOM in endosomal trafficking establish intrinsic cell polarity in the Arabidopsis stomatal lineage.Funding Information
- National Natural Science Foundation of China (31771515, 31970804)
- MEXT | Japan Science and Technology Agency (JPMJCR14M5)
- Howard Hughes Medical Institute
- U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (GM109080, GM131827)
- U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences
- National Science Foundation (1851907, 1952823)
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