Effect of heat stress on oxidative damage and antioxidant defense system in white clover (Trifolium repens L.)
- 21 October 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Planta
- Vol. 254 (5), 1-17
- https://doi.org/10.1007/s00425-021-03751-9
Abstract
Main conclusion This study leads to advances in the field of heat tolerance among different plant species. We concluded that a coordinated, increased antioxidant defense system enabled white clover to reduce heat-induced oxidative damage. Abstract The rise in global ambient temperature has a wide range of effects on plant growth, and, therefore, on the activation of various molecular defenses before the appearance of heat damage. Elevated temperatures result in accelerated generation of reactive oxygen species (ROS), causing an imbalance between ROS production and the ability of scavenging systems to detoxify and remove the reactive intermediates. The aim of this study was to determine the role of antioxidant defense systems in the alleviation of heat stress (HS) consequences in white clover (Trifolium repens L.), which is cultivated worldwide. We evaluated how temperature and time parameters contribute to the thermotolerance of white clover at different growth stages. We revealed HS protection in white clover from 37 to 40 °C, with 40 °C providing the greatest protection of 3-day-old seedlings and 28-day-old adult plants. Heat-provoked oxidative stress in white clover was confirmed by substantial changes in electrolyte leakage, malondialdehyde (MDA), and chlorophyll content, as well as superoxide anion (O2·−) and hydrogen peroxide (H2O2) production. Furthermore, superoxide dismutase (SOD) and ascorbate peroxidase (APX) as well as a high level of GSH non-enzymatic antioxidant were the most responsive, and were associated with acquired thermotolerance through the regulation of ROS generation. We demonstrated, by studying protoplast transient gene expression, direct genetic evidence of endogenous antioxidant-related genes that confer HS tolerance in white clover. Our present study clearly establishes that oxidative stress ensues from HS, which triggers the induction of antioxidant defense systems for ROS scavenging in white clover.Keywords
Funding Information
- Ministry of Science and Technology, Taiwan (105‐2311‐B‐024‐001, 107‐2311‐B‐024‐001)
- Ministry of Education (PAG107012)
This publication has 83 references indexed in Scilit:
- Physiological, Biochemical, and Molecular Mechanisms of Heat Stress Tolerance in PlantsInternational Journal of Molecular Sciences, 2013
- Plant tolerance to high temperature in a changing environment: scientific fundamentals and production of heat stress-tolerant cropsFrontiers in Plant Science, 2013
- Heat perception and signalling in plants: a tortuous path to thermotoleranceNew Phytologist, 2010
- Recovery of heat shock-triggered released apoplastic Ca2+ accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlingsJournal of Experimental Botany, 2010
- Cytosol-Localized Heat Shock Factor-Binding Protein, AtHSBP, Functions as a Negative Regulator of Heat Shock Response by Translocation to the Nucleus and Is Required for Seed Development in ArabidopsisPlant Physiology, 2010
- Reactive Species and Antioxidants. Redox Biology Is a Fundamental Theme of Aerobic LifePlant Physiology, 2006
- The Heat Stress Transcription Factor HsfA2 Serves as a Regulatory Amplifier of a Subset of Genes in the Heat Stress Response in ArabidopsisPlant Molecular Biology, 2006
- Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compoundsPlanta, 1999
- The role of active oxygen in the response of plants to water deficit and desiccationNew Phytologist, 1993
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970