New Search

Export article
Open Access

Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework

Ping Song, Juwen Shen, Dekai Ye, Baijun Dong, Fei Wang, Hao Pei, Jianbang Wang, , Lihua Wang, Wei Xue, Yiran Huang, Gang Huang, ,
Nature Communications , Volume 11, pp 1-10; doi:10.1038/s41467-020-14664-8

Abstract: Protein-protein interactions are spatially regulated in living cells to realize high reaction efficiency, as seen in naturally existing electron-transfer chains. Nevertheless, arrangement of chemical/biochemical components at the artificial device interfaces does not possess the same level of control. Here we report a tetrahedral DNA framework-enabled bulk enzyme heterojunction (BEH) strategy to program the multi-enzyme catalytic cascade at the interface of electrochemical biosensors. The construction of interpenetrating network of BEH at the millimeter-scale electrode interface brings enzyme pairs within the critical coupling length (CCL) of ~10 nm, which in turn greatly improve the overall catalytic cascade efficiency by ~10-fold. We demonstrate the BEH generality with a range of enzyme pairs for electrochemically detecting clinically relevant molecular targets. As a proof of concept, a BEH-based sarcosine sensor enables single-step detection of the metabolic biomarker of sarcosine with ultrasensitivity, which hold the potential for precision diagnosis of early-stage prostate cancer.
Keywords: DNA nanotechnology / Bioanalytical chemistry

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

Share this article

Click here to see the statistics on "Nature Communications" .
References (48)
    Cited by 17 articles
      Back to Top Top