Peripheral blood CD163(+) monocytes and soluble CD163 in dry and neovascular age‐related macular degeneration
Open Access
- 25 April 2020
- journal article
- research article
- Published by Wiley in The FASEB Journal
- Vol. 34 (6), 8001-8011
- https://doi.org/10.1096/fj.201901902rr
Abstract
Macrophages are the main infiltrating immune cells in choroidal neovascularization (CNV), a hallmark of the human wet, or neovascular age-related macular degeneration (AMD). Due to their plasticity and ability to adapt to the local microenvironment in a tissue-dependent manner, macrophages display polar functional phenotypes characterized by their cell surface markers and their cytokine profiles. We found accumulation of hemoglobin-scavenging cluster of differentiation 163 (CD163)(+) macrophages in laser-induced CNV lesions and higher expression of CD163(+) monocytes in the peripheral blood on day 7 post injury in mice. In comparison, CD80(+) macrophages did not differ with laser-injury in young or aged mice and did not significantly change in the peripheral blood of CNV mice. We examined the percentages of CD163(+), CD206(+), and CD80(+) monocytes in the peripheral blood of patients with wet AMD, patients with dry AMD, and in age-matched individuals without AMD as controls. Percentages of peripheral blood CD163(+) monocytes in both dry AMD (P < .001) and wet AMD (P < .05) were higher than in age-matched non-AMD controls, while there was no difference between the groups in the percentages of peripheral CD206(+) and CD80(+) monocytes. Further, serum level of soluble CD163 (sCD163) was elevated only in patients with wet AMD (P < .05). An examination of 40 cytokine levels across the study groups revealed that anti-VEGF treated patients with wet AMD, who showed no exudative signs on the day of blood drawing had a cytokine profile that was similar to that of non-AMD individuals. These results indicate that CD163 could be further evaluated for its potential as a useful marker of disease activity in patients with neovascular AMD. Future studies will address the origin and potential mechanistic role of CD163(+) macrophages in wet AMD pathologies of angiogenesis and leakage of blood components.Keywords
Funding Information
- Juvenile Diabetes Research Foundation United States of America (Innovation award)
This publication has 41 references indexed in Scilit:
- Retinal Vascular Caliber as a Biomarker for Diabetes Microvascular ComplicationsDiabetes Care, 2013
- Retinal pigment epithelium response to oxidant injury in the pathogenesis of early age-related macular degenerationMolecular Aspects of Medicine, 2012
- Macrophage polarization in the maculae of age‐related macular degeneration: A pilot studyPathology International, 2011
- Apical-to-Basolateral Transcytosis of Photoreceptor Outer Segments Induced by Lipid Peroxidation Products in Human Retinal Pigment Epithelial CellsInvestigative Ophthalmology & Visual Science, 2010
- Identification of Splenic Reservoir Monocytes and Their Deployment to Inflammatory SitesScience, 2009
- Macrophages in neovascular age-related macular degeneration: friends or foes?Eye, 2008
- Immunopathological aspects of age-related macular degenerationSeminars in Immunopathology, 2008
- Senescence regulates macrophage activation and angiogenic fate at sites of tissue injury in miceJCI Insight, 2007
- Complement Factor H Polymorphism in Age-Related Macular DegenerationScience, 2005
- Functional plasticity of macrophages: reversible adaptation to changing microenvironmentsJournal of Leukocyte Biology, 2004