Rapid detection of carbamate pesticide residues using microchip electrophoresis combining amperometric detection

Publisher Website
Google Scholar
Abstract
The long-term consumption of food with pesticide residues has harmful effects on human health and the demand for pesticide detection technology tends to be miniaturized and instant. To this end, we demonstrated the first application of indirectly detecting two carbamate pesticides, metolcarb and carbaryl, by gold nanoparticle–modified indium tin oxide electrode in dual-channel microchip electrophoresis and amperometric detection (ME-AD) system. m-Cresol and α-naphthol were obtained after pesticide hydrolysis in alkaline solution, and then separated and detected by ME-AD. Parameters including the detection potential and running buffer concentration and pH were optimized to improve the detection sensitivity and separation efficiency. Under the optimal conditions, the two analytes were completely separated within 80 s. m-Cresol and α-naphthol presented a wide linear range from 1 to 100 μM, with limits of detection of 0.16 μM and 0.34 μM, respectively (S/N = 3). Moreover, the reliability of this system was demonstrated by analyzing metolcarb and carbaryl in spiked vegetable samples. Graphical abstract
Funding Information
  • the Hunan Provincial Innovation Foundation for Postgraduate (CX20190246)
  • the Natural Science Foundation of Hunan Province (2020JJ9020)
  • the Fundamental Research Funds for the Central Universities of Central South University (1053320190355)
  • The National Natural Science Foundation of China (81311140268)
  • The Science and Technology Program of Hunan Province (2017SK2164)
  • the Shenzhen Science and Technology Innovation Committee of China (JCYJ20170413105329648)

This publication has 36 references indexed in Scilit: