Grazing Affects Bacterial and Fungal Diversities and Communities in the Rhizosphere and Endosphere Compartments of Leymus chinensis through Regulating Nutrient and Ion Distribution
Open Access
- 25 February 2021
- journal article
- research article
- Published by MDPI AG in Microorganisms
- Vol. 9 (3), 476
- https://doi.org/10.3390/microorganisms9030476
Abstract
Plant-associated endophytic microorganisms are essential to developing successful strategies for sustainable agriculture. Grazing is an effective practice of grassland utilization through regulating multitrophic relationships in natural grasslands. This study was conducted for exploring the effects of grazing on the diversities and communities of bacteria and fungi presented in rhizosphere soils, roots, stems, and leaves of Leymus chinensis (L. chinensis), based on high-throughput sequencing. Grazing increased bacterial diversity but reduced fungal diversity in plant leaves. Further analysis confirmed that the abundance of Chloroflexi, Gemmatimonadota, Nitrospirota, Sordariales, and Pezizales in plant leaves was increased by grazing. The Bray–Curtis similarities of microbial communities in the endosphere were higher under grazing plots than non-grazing plots. Moreover, the bacterial communities were significantly correlated with ions, while the nutrient and negative ions exhibited strong influence on fungal communities. We concluded that grazing-induced changes of microbial diversities and communities in different compartments of a dominant perennial grass (L. chinensis) could be attributed to the nutrient and ion distribution in host plant. The current study highlights the importance of livestock in mediating diversities and communities of endophytic microbes, and will be useful for better understanding the complexity of multitrophic interactions in a grassland ecosystem.Keywords
Funding Information
- Deli Wang (National Natural Science Foundation of China (31770520), National Natural Science Foundation of China (41807052), National Natural Science Foundation of China (32061143027), Grassland Management Project of State Forestry and Grassland Administration (202004202039))
This publication has 70 references indexed in Scilit:
- Temporal variations in endophytic fungal assemblages of Ginkgo biloba L.Journal of Forest Research, 2012
- Using network analysis to explore co-occurrence patterns in soil microbial communitiesThe ISME Journal, 2011
- QIIME allows analysis of high-throughput community sequencing dataNature Methods, 2010
- Enhanced drought tolerance in transgenic Leymus chinensis plants with constitutively expressed wheat TaLEA 3Biotechnology Letters, 2008
- Ecology of meadow steppe in northeast ChinaThe Rangeland Journal, 2008
- Bacterial endophytes: recent developments and applicationsFEMS Microbiology Letters, 2008
- Soil Attributes in a Sierra Nevada Riparian Meadow as Influenced by GrazingRangeland Ecology & Management, 2006
- Influences of continuous grazing and livestock exclusion on soil properties in a degraded sandy grassland, Inner Mongolia, northern ChinaCATENA, 2005
- Colonisation of poplar trees by gfp expressing bacterial endophytesFEMS Microbiology Ecology, 2004
- Tropical Soil Biology and FertilitySoil Science, 1994