Molecular evolution and characterization of hemagglutinin and neuraminidase of influenza A(H1N1)pdm09 viruses isolated in Beijing, China, during the 2017–2018 and 2018–2019 influenza seasons
- 3 November 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 166 (1), 179-189
- https://doi.org/10.1007/s00705-020-04869-z
Abstract
We investigated and analysed the molecular evolution of hemagglutinin (HA) and neuraminidase (NA) of influenza A(H1N1)pdm09 virus during the 2017–2018 and 2018–2019 influenza seasons in Beijing, China. We collected and extracted RNA from influenza A(H1N1)pdm09 strains from Peking University People’s Hospital and analyzed their HA and NA genes by RT-PCR and sequencing. Phylogenetic analysis of HA and NA sequences was used to compare the amino acid sequences of 51 strains with those of reference strains. All strains belonged to subclade 6B.1, with S162N and I216T substitutions (H1 numbering). Our strains differed from strain A/Michigan/45/2015, with the substitutions S91R, S181T and I312V in the HA antigenic epitope. An E189G mutation was detected in the 190 helix of the receptor binding region of HA. A new potential glycosylation site, 179 (NQT), which was not detected before the 2015 influenza season, was identified. Two strains were mutated at I223, the NA inhibitor resistance site. During 2012-2019, amino acids of HA and NA mutated over time. Co-occurrence mutations N146D, S200P, S202I and A273T in HA appeared along with Q51K, F74S and D416N in NA in six strains during two influenza seasons. Our work reveals the molecular changes and phylogenetic characteristics of influenza A(H1N1)pdm09 virus and suggests that a vaccine probably provides suboptimal protection. The biological characteristics of the new glycosylation and drug-resistance sites detected in this work need to be studied further. The co-occurrence of mutations in HA and NA might affect the characteristics of the virus and need to be given more attention.Funding Information
- National Natural Science Foundation of China (81541139)
This publication has 32 references indexed in Scilit:
- Epidemiological and genetic characterization of pH1N1 and H3N2 influenza viruses circulated in MENA region during 2009–2017BMC Infectious Diseases, 2019
- Seasonal Genetic Drift of Human Influenza A Virus Quasispecies Revealed by Deep SequencingFrontiers in Microbiology, 2018
- Revision of clinical case definitions: influenza-like illness and severe acute respiratory infectionBulletin of the World Health Organization, 2017
- Interim estimates of 2015/16 vaccine effectiveness against influenza A(H1N1)pdm09, Canada, February 2016Eurosurveillance, 2016
- Molecular epidemiology and evolution of influenza A and B viruses during winter 2013-2014 in Beijing, ChinaArchiv für die gesamte Virusforschung, 2015
- Molecular epidemiology and evolution of A(H1N1)pdm09 and H3N2 virus during winter 2012–2013 in Beijing, ChinaInfection, Genetics and Evolution, 2014
- Impact of Potential Permissive Neuraminidase Mutations on Viral Fitness of the H275Y Oseltamivir-Resistant Influenza A(H1N1)pdm09 VirusIn Vitro, in Mice and in FerretsJournal of Virology, 2014
- Comparative Dynamics and Distribution of Influenza Drug Resistance Acquisition to Protein M2 and Neuraminidase InhibitorsMolecular Biology and Evolution, 2013
- Influenza neuraminidaseInfluenza and Other Respiratory Viruses, 2011
- Clinical and virological course of infection with haemagglutinin D222G mutant strain of 2009 pandemic influenza A (H1N1) virusJournal of Clinical Virology, 2011