Circ_0006948 Contributes to Cell Growth, Migration, Invasion and Epithelial–Mesenchymal Transition in Esophageal Carcinoma
- 25 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Digestive Diseases and Sciences
- Vol. 67 (2), 492-503
- https://doi.org/10.1007/s10620-021-06894-7
Abstract
Background Circular RNAs (circRNAs) can act as promoters or inhibitors in cancer progression. Has_circ_0006948 (circ_0006948) was reported to aggravate the malignant behaviors of esophageal carcinoma (EC). Aims This study focused on investigating the molecular mechanism of circ_0006948 in EC progression. Methods The quantitative real-time polymerase chain reaction was performed to detect the expression of circ_0006948, microRNA-4262 (miR-4262) and fibronectin type III domain containing 3B (FNDC3B). Cell growth analysis was conducted by Cell Counting Kit-8 and colony formation assays. Cell migration and invasion were assessed by transwell assay. Epithelial–mesenchymal transition (EMT)-associated proteins and FNDC3B protein expression were assayed using western blot. Dual-luciferase reporter and RNA pull-down assays were performed to validate the target combination. Xenograft tumor assay was used for investigating the role of circ_0006948 in vivo. Results Circ_0006948 was upregulated in EC tissues and cells. Downregulating the expression of circ_0006948 or FNDC3B repressed cell growth, migration, invasion and EMT in EC cells. Target analysis indicated that miR-4262 was a target for circ_0006948 and FNDC3B was a downstream gene for miR-4262. Moreover, circ_0006948 could affect the expression of FNDC3B via sponging miR-4262. The effects of si-circ_0006948#1 on EC cells were partly restored by miR-4262 inhibition or FNDC3B overexpression. In addition, circ_0006948 also facilitated EC tumorigenesis in vivo by targeting the miR-4262/FNDC3B axis. Conclusion Taken together, circ_0006948 functioned as an oncogenic factor in EC by the miR-4262-mediated FNDC3B expression regulation.Keywords
This publication has 42 references indexed in Scilit:
- Immune Therapeutics in the Treatment of Advanced Gastric and Esophageal CancerAnticancer Research, 2018
- Circular RNAs Act as miRNA SpongesPublished by Springer Science and Business Media LLC ,2018
- Upregulation of MicroRNA-4262 Targets Kaiso (ZBTB33) to Inhibit the Proliferation and EMT of Cervical Cancer CellsOncology Research, 2018
- CiRS-7 promotes growth and metastasis of esophageal squamous cell carcinoma via regulation of miR-7/HOXB13Cell Death & Disease, 2018
- Molecular landscape of esophageal cancer: implications for early detection and personalized therapyAnnals of the New York Academy of Sciences, 2018
- Surgical treatment of esophageal cancer in the era of multimodality managementAnnals of the New York Academy of Sciences, 2018
- FNDC3B promotes epithelial-mesenchymal transition in tongue squamous cell carcinoma cells in a hypoxic microenvironmentOncology Reports, 2018
- Tumor suppressor microRNA-34a inhibits cell migration and invasion by targeting MMP-2/MMP-9/FNDC3B in esophageal squamous cell carcinomaInternational Journal of Oncology, 2017
- An integrated analysis of the effects of microRNA and mRNA on esophageal squamous cell carcinomaMolecular Medicine Reports, 2015
- Esophageal cancer: Risk factors, screening and endoscopic treatment in Western and Eastern countriesWorld Journal of Gastroenterology, 2015