Adsorptive granulomonocytapheresis alters the gut bacterial microbiota in patients with active ulcerative colitis
- 18 February 2021
- journal article
- research article
- Published by Wiley in Journal of Clinical Apheresis
- Vol. 36 (3), 454-464
- https://doi.org/10.1002/jca.21887
Abstract
Background Ulcerative colitis (UC) is a refractory disease with unclear etiology. Studies have shown that UC is closely associated with gut microbiota dysbiosis. Adsorptive granulomonocytapheresis (GMA) using an Adacolumn has been found to treat UC effectively, but its underlying mechanism of treatment has not been fully elucidated. In this study, we aimed to investigate the influence of GMA on the gut microbiota in patients with active UC. Methods We conducted a single‐center prospective analysis of patients with active UC who received GMA therapy and ultimately achieved clinical remission. Stool samples of healthy controls and patients before and after 5 or 10 sessions of GMA therapy were collected. Subsequently, high‐throughput sequencing of the 16S rRNA V3 and V4 gene region of the stool was conducted and clustering of operational taxonomic units and species annotation were performed. Results Gut microbial profiles in patients with UC were characterized by low bacterial diversity. After 5 or 10 sessions of GMA therapy, the gut microbiota diversity in patients with UC increased and was similar to that of healthy controls. UC was further characterized by increased abundances of Proteobacteria and Bacteroides, as well as decreased abundances of Faecalibacterium, Roseburia, Firmicutes, and Dialister; however, after GMA therapy, the abundance of Bacteroides decreased, whereas those of Faecalibacterium, Roseburia, and Firmicutes increased. Conclusions Active UC is associated with gut microbiota dysbiosis. GMA therapy exerts a strong regulatory effect on the gut microbiota in patients with UC.Keywords
This publication has 56 references indexed in Scilit:
- A decrease of the butyrate-producing speciesRoseburia hominisandFaecalibacterium prausnitziidefines dysbiosis in patients with ulcerative colitisGut, 2013
- The Microbial Metabolites, Short-Chain Fatty Acids, Regulate Colonic T reg Cell HomeostasisScience, 2013
- Structure, function and diversity of the healthy human microbiomeNature, 2012
- Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatmentGenome Biology, 2012
- Disease phenotype and genotype are associated with shifts in intestinal-associated microbiota in inflammatory bowel diseasesInflammatory Bowel Diseases, 2011
- Early life: gut microbiota and immune development in infancyBeneficial Microbes, 2010
- QIIME allows analysis of high-throughput community sequencing dataNature Methods, 2010
- A human gut microbial gene catalogue established by metagenomic sequencingNature, 2010
- Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseasesProceedings of the National Academy of Sciences of the United States of America, 2007
- Diversity of the Human Intestinal Microbial FloraScience, 2005