Isolation of Circulating Tumor Cells from Glioblastoma Patients by Direct Immunomagnetic Targeting
Open Access
- 11 May 2020
- journal article
- research article
- Published by MDPI AG in Applied Sciences
- Vol. 10 (9), 3338
- https://doi.org/10.3390/app10093338
Abstract
Glioblastoma (GBM) is the most common form of primary brain cancer in adults and tissue biopsies for diagnostic purposes are often inaccessible. The postulated idea that brain cancer cells cannot pass the blood–brain barrier to form circulating tumor cells (CTCs) has recently been overthrown and CTCs have been detected in the blood of GBM patients albeit in low numbers. Given the potential of CTCs to be analyzed for GBM biomarkers that may guide therapy decisions it is important to define methods to better isolate these cells. Here, we determined markers for immunomagnetic targeting and isolation of GBM-CTCs and confirmed their utility for CTC isolation from GBM patient blood samples. Further, we identified a new marker to distinguish isolated GBM-CTCs from residual lymphocytes.Funding Information
- Cancer Institute NSW (13/TRC/1-01)
This publication has 21 references indexed in Scilit:
- Alex's Lemonade Stand Foundation Infant and Childhood Primary Brain and Central Nervous System Tumors Diagnosed in the United States in 2007–2011Neuro-Oncology, 2014
- Brain Tumor Cells in Circulation Are Enriched for Mesenchymal Gene ExpressionCancer Discovery, 2014
- CBTRUS Statistical Report: Primary Brain and Central Nervous System Tumors Diagnosed in the United States in 2007-2011Neuro-Oncology, 2014
- Detection of Brain Tumor Cells in the Peripheral Blood by a Telomerase Promoter-Based AssayCancer Research, 2014
- Glioblastoma and Other Malignant GliomasJAMA, 2013
- New frontiers in circulating tumor cell analysis: A reference guide for biomolecular profiling toward translational clinical useInternational Journal of Cancer, 2013
- Melanocytes: A Window into the Nervous SystemJournal of Investigative Dermatology, 2012
- Epigenetic regulation of glial fibrillary acidic protein by DNA methylation in human malignant gliomasNeuro-Oncology, 2010
- Circulating Tumor Cells Predict Survival Benefit from Treatment in Metastatic Castration-Resistant Prostate CancerClinical Cancer Research, 2008
- Circulating Tumor Cells at Each Follow-up Time Point during Therapy of Metastatic Breast Cancer Patients Predict Progression-Free and Overall SurvivalClinical Cancer Research, 2006