A novel micellar formulation based on natural plant extracts enhances the efficacy of hydrogen peroxide against biofilms of Staphylococcus spp. and Pseudomonas aeruginosa
- 27 May 2020
- journal article
- research article
- Published by Taylor & Francis Ltd in Biofouling
- Vol. 36 (5), 576-586
- https://doi.org/10.1080/08927014.2020.1782388
Abstract
The antibacterial efficacy of hydrogen peroxide encapsulated in micelles (mH2O2) against biofilms was compared with that of hydrogen peroxide alone and of three commercially available aqueous biocides. The activity of mH2O2 on 24-h biofilms of reference strains of Staphylococcus spp. and Pseudomonas aeruginosa was tested in a static microtiter plate model. The biofilms were incubated with mH2O2 (17% v/v H2O2, 2% lactic acid, 0.3% phytoextract, H2O) and its individual ingredients and compared with three aqueous biocides at different concentrations and times of exposure. After 5-min exposure, 10% mH2O2 (corresponding to 1.7% v/v H2O2) achieved > 8 log10 reductions against all the test strains, while 1.7% H2O2 achieved a maximum of 1.5 log10 reduction. After 5-min exposure, none of the commercially available biocides tested showed themselves to be capable of completely eliminating the test strains embedded in biofilms. Hydrogen peroxide encapsulated in micelles demonstrated enhanced activity against planktonic cells and biofilms of Staphylococcus spp. and P. aeruginosa.Keywords
This publication has 24 references indexed in Scilit:
- Polymeric micellar nanoplatforms for Fenton reaction as a new class of antibacterial agentsJournal of Controlled Release, 2015
- The Importance of Clinical Surveillance in Detecting Legionnaires' Disease Outbreaks: A Large Outbreak in a Hospital With a Legionella Disinfection System—Pennsylvania, 2011–2012Clinical Infectious Diseases, 2015
- Differential Bacterial Load on Components of Total Knee Prosthesis in Patients with Prosthetic Joint InfectionThe International Journal of Artificial Organs, 2012
- Microtiter Dish Biofilm Formation AssayJournal of Visualized Experiments, 2011
- Challenging the Sterrad 100NX sterilizer with different carrier materials and wrappings under experimental “clean” and “dirty” conditionsAmerican Journal of Infection Control, 2010
- Modality of bacterial growth presents unique targets: how do we treat biofilm-mediated infections?Current Opinion in Microbiology, 2010
- Effects of Azithromycin in Combination with Vancomycin, Daptomycin, Fosfomycin, Tigecycline, and Ceftriaxone on Staphylococcus epidermidis BiofilmsAntimicrobial Agents and Chemotherapy, 2009
- Effects of alcohols, povidone-iodine and hydrogen peroxide on biofilms of Staphylococcus epidermidisJournal of Antimicrobial Chemotherapy, 2007
- Biofilms: Survival Mechanisms of Clinically Relevant MicroorganismsClinical Microbiology Reviews, 2002
- Heterogeneity in biofilms: Table 1FEMS Microbiology Reviews, 2000