Injection of Porcine Adipose Tissue-Derived Stroma Cells via Waterjet Technology
- 23 November 2021
- journal article
- research article
- Published by MyJove Corporation in Journal of Visualized Experiments
- No. 177,p. e63132
- https://doi.org/10.3791/63132
Abstract
Urinary incontinence (UI) is a highly prevalent condition characterized by the deficiency of the urethral sphincter muscle. Regenerative medicine branches, particularly cell therapy, are novel approaches to improve and restore the urethral sphincter function. Even though injection of active functional cells is routinely performed in clinical settings by needle and syringe, these approaches have significant disadvantages and limitations. In this context, needle-free waterjet (WJ) technology is a feasible and innovative method that can inject viable cells by visual guided cystoscopy in the urethral sphincter. In the present study, we used WJ to deliver porcine adipose tissue-derived stromal cells (pADSCs) into cadaveric urethral tissue and subsequently investigated the effect of WJ delivery on cell yield and viability. We also assessed the biomechanical features (i.e., elasticity) by atomic force microscopy (AFM) measurements. We showed that WJ delivered pADSCs were significantly reduced in their cellular elasticity. The viability was significantly lower compared to controls but is still above 80%.This publication has 34 references indexed in Scilit:
- Microfluidic jet injection for delivering macromolecules into cellsJournal of Micromechanics and Microengineering, 2013
- A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosisThe Journal of cell biology, 2012
- The Interaction between Nesprins and Sun Proteins at the Nuclear Envelope Is Critical for Force Transmission between the Nucleus and CytoskeletonOnline Journal of Public Health Informatics, 2011
- Lamin A variants that cause striated muscle disease are defective in anchoring transmembrane actin-associated nuclear lines for nuclear movementProceedings of the National Academy of Sciences of the United States of America, 2010
- Bone marrow mesenchymal stromal cell therapy for external urethral sphincter restoration in a rat model of stress urinary incontinenceNeurourology and Urodynamics, 2010
- Myoblasts Differentiated From Adipose-derived Stem Cells to Treat Stress Urinary IncontinenceUrology, 2010
- Viscoelastic properties of human mesenchymally-derived stem cells and primary osteoblasts, chondrocytes, and adipocytesJournal of Biomechanics, 2008
- Elasticity measurement of living cells with an atomic force microscope: data acquisition and processingPflügers Archiv - European Journal of Physiology, 2008
- Atomic force microscopy probing of cell elasticityMicron, 2007
- Cardiomyocyte Grafting for Cardiac Repair: Graft Cell Death and Anti-Death StrategiesJournal of Molecular and Cellular Cardiology, 2001