Growth of Haematococcus pluvialis on a Small-Scale Angled Porous Substrate Photobioreactor for Green Stage Biomass
Open Access
- 18 February 2021
- journal article
- research article
- Published by MDPI AG in Applied Sciences
- Vol. 11 (4), 1788
- https://doi.org/10.3390/app11041788
Abstract
In the production of astaxanthin from Haematococcus pluvialis, the process of growing algal biomass in the vegetative green stage is an indispensable step in both suspended and immobilized cultivations. The green algal biomass is usually cultured in a suspension under a low light intensity. However, for astaxanthin accumulation, the microalgae need to be centrifuged and transferred to a new medium or culture system, a significant difficulty when upscaling astaxanthin production. In this research, a small-scale angled twin-layer porous substrate photobioreactor (TL-PSBR) was used to cultivate green stage biomass of H. pluvialis. Under low light intensities of 20–80 µmol photons m−2·s−1, algae in the biofilm consisted exclusively of non-motile vegetative cells (green palmella cells) after ten days of culturing. The optimal initial biomass density was 6.5 g·m−2, and the dry biomass productivity at a light intensity of 80 µmol photons m−2·s−1 was 6.5 g·m−2·d−1. The green stage biomass of H. pluvialis created in this small-scale angled TL-PSBR can be easily harvested and directly used as the source of material for the inoculation of a pilot-scale TL-PSBR for the production of astaxanthin.Keywords
This publication has 38 references indexed in Scilit:
- Microalgal biofilms: A further step over current microalgal cultivation techniquesScience of The Total Environment, 2018
- Effect of red cyst cell inoculation and iron(II) supplementation on autotrophic astaxanthin production by Haematococcus pluvialis under outdoor summer conditionsJournal of Biotechnology, 2016
- Factors affecting algae biofilm growth and lipid production: A reviewRenewable and Sustainable Energy Reviews, 2015
- Biofilm-based algal cultivation systemsApplied Microbiology and Biotechnology, 2015
- Microalgal biofilms for biomass productionJournal of Applied Phycology, 2014
- Attached cultivation of Haematococcus pluvialis for astaxanthin productionBioresource Technology, 2014
- Determination of the time transferring cells for astaxanthin production considering two-stage process of Haematococcuspluvialis cultivationBioresource Technology, 2011
- On the relative efficiency of two‐ vs. one‐stage production of astaxanthin by the green alga Haematococcus pluvialisBiotechnology & Bioengineering, 2007
- Astaxanthin biosynthesis from simultaneous N and P uptake by the green alga Haematococcus pluvialis in primary-treated wastewaterBiochemical Engineering Journal, 2006
- A novel double-layered photobioreactor for simultaneous Haematococcus pluvialis cell growth and astaxanthin accumulationJournal of Biotechnology, 2006