Ectopic Expression of AhGLK1b (GOLDEN2-like Transcription Factor) in Arabidopsis Confers Dual Resistance to Fungal and Bacterial Pathogens
Open Access
- 23 March 2020
- Vol. 11 (3), 343
- https://doi.org/10.3390/genes11030343
Abstract
GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low calcium with a complete open reading frame (ORF) of 1212 bp. The AhGLK1b has 99.26% and 96.28% sequence similarities with its orthologs in Arachis ipaensis and A. duranensis, respectively. In the peanut, the AhGLK1b was localized in the nucleus and demonstrated the highest expression in the leaf, followed by the embryo. Furthermore, the expression of AhGLK1b was induced significantly in response to a bacterial pathogen, Ralstonia solanacearum infection. Ectopic expression of AhGLK1b in Arabidopsis showed stronger resistance against important phytopathogenic fungi S. sclerotiorum. It also exhibited high resistance to infection of the bacterial pathogen Pst DC3000. AhGLK1b-expressing Arabidopsis induced defense-related genes including PR10 and Phox/Bem 1 (PBI), which are involved in multiple disease resistance. Taken together, the results suggest that AhGLK1b might be useful in providing dual resistance to fungal and bacterial pathogens as well as tolerance to abiotic stresses.Funding Information
- National Natural Science Foundation of China (31601337, U1705233)
This publication has 73 references indexed in Scilit:
- Network Inference Analysis Identifies an APRR2-Like Gene Linked to Pigment Accumulation in Tomato and Pepper FruitsPlant Physiology, 2013
- Nuclear jasmonate and salicylate signaling and crosstalk in defense against pathogensFrontiers in Plant Science, 2013
- Evolution of GOLDEN2-LIKE gene function in C3 and C4 plantsPlanta, 2012
- Ectopic Overexpression of The Transcription Factor OsGLK1 Induces Chloroplast Development in Non-Green Rice CellsPlant and Cell Physiology, 2009
- GLK Transcription Factors Coordinate Expression of the Photosynthetic Apparatus in ArabidopsisTHE PLANT CELL ONLINE, 2009
- Phosphate Starvation Responses and Gibberellic Acid Biosynthesis are Regulated by the MYB62 Transcription Factor in ArabidopsisMolecular Plant, 2009
- Analyzing real-time PCR data by the comparative CT methodNature Protocols, 2008
- Global expression analysis of nucleotide binding site-leucine rich repeat-encoding and related genes in ArabidopsisBMC Plant Biology, 2007
- The GLK1 ‘regulon’ encodes disease defense related proteins and confers resistance to Fusarium graminearum in ArabidopsisBiochemical and Biophysical Research Communications, 2007
- Index of plant hosts of Sclerotinia sclerotiorumCanadian Journal of Plant Pathology, 1994