New Search

Export article
Open Access

Effects of in vitro short- and long-term treatment with telomerase inhibitor in U-251 glioma cells

Tales Henrique Andrade da Mota, Ana Flávia Reis Guimarães, Amandda Évelin Silva de Carvalho, Felipe Saldanha de Araujo, Giselle Pinto De Faria Lopes, Fábio Pittella-Silva, , Diêgo Madureira de Oliveira
Published: 21 December 2021
 by  IOS Press

Abstract: BACKGROUND: The inhibition of the enzyme telomerase (TERT) has been widely investigated as a new pharmacological approach for cancer treatment, but its real potential and the biochemical consequences are not totally understood. OBJECTIVE: Here, we investigated the effects of the telomerase inhibitor MST-312 on a human glioma cell line after both short- and long-term (290 days) treatments. METHODS: Effects on cell growth, viability, cell cycle, morphology, cell death and genes expression were assessed. RESULTS: We found that short-term treatment promoted cell cycle arrest followed by apoptosis. Importantly, cells with telomerase knock-down revealed that the toxic effects of MST-312 are partially TERT dependent. In contrast, although the long-term treatment decreased cell proliferation at first, it also caused adaptations potentially related to treatment resistance and tumor aggressiveness after long time of exposition. CONCLUSIONS: Despite the short-term effects of telomerase inhibition not being due to telomere erosion, they are at least partially related to the enzyme inhibition, which may represent an important strategy to pave the way for tumor growth control, especially through modulation of the non-canonical functions of telomerase. On the other hand, long-term exposure to the inhibitor had the potential to induce cell adaptations with possible negative clinical implications.
Keywords: treatment / cell death / genes expression / adaptations / telomerase / apoptosis / viability / enzyme inhibition / inhibitor / functions

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

Share this article

Click here to see the statistics on "Tumor Biology" .
References (39)
    Back to Top Top