SalmonellaPersistence and Host Immunity Are Dictated by the Anatomical Microenvironment
- 21 July 2020
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 88 (8)
- https://doi.org/10.1128/iai.00026-20
Abstract
The intracellular bacterial pathogen Salmonella is able to evade the immune system and persist within the host. In some cases, these persistent infections are asymptomatic for long periods and represent a significant public health hazard as potential chronic carriers, yet the mechanisms that control persistence are incompletely understood. Using a mouse model of chronic typhoid fever combined with MHC class II tetramers to interrogate endogenous, Salmonella-specific CD4+ helper T cells, we show certain host microenvironments may favorably contribute to a pathogen's ability to persist in vivo. We demonstrate that the environment in the hepatobiliary system may contribute to the persistence of Salmonella Typhimurium through liver resident immunoregulatory CD4+ helper T cells, alternatively activated macrophages, and impaired bactericidal activity. This contrasts with lymphoid organs, such as the spleen and mesenteric lymph nodes, where these same cells appear to have a greater capacity for bacterial killing, which may contribute to control of bacteria in these organs. We also find that, following an extended period of infection of greater than two years, the liver appeared to be the only site that harbored Salmonella. This work establishes a potential role for non-lymphoid organ immunity in regulating chronic bacterial infections and provides further evidence for the hepatobiliary system as the site of chronic Salmonella infection.Keywords
Funding Information
- HHS | National Institutes of Health (R01 AI103343)
- HHS | National Institutes of Health (R01 AI116917)
This publication has 82 references indexed in Scilit:
- A Mouse Model of Salmonella Typhi InfectionCell, 2012
- Dissemination of Persistent Intestinal Bacteria via the Mesenteric Lymph Nodes Causes Typhoid RelapseInfection and Immunity, 2011
- Chronic and acute infection of the gall bladder by Salmonella Typhi: understanding the carrier stateNature Reviews Microbiology, 2010
- A Mouse Model for the Human Pathogen Salmonella TyphiCell Host & Microbe, 2010
- Gallstones play a significant role inSalmonellaspp. gallbladder colonization and carriageProceedings of the National Academy of Sciences of the United States of America, 2010
- Mesenteric Lymph Nodes Confine Dendritic Cell-Mediated Dissemination ofSalmonella entericaSerovar Typhimurium and Limit Systemic Disease in MiceInfection and Immunity, 2009
- Tracking epitope-specific T cellsNature Protocols, 2009
- Dendritic Cell Antigen Presentation Drives Simultaneous Cytokine Production by Effector and Regulatory T Cells in Inflamed SkinImmunity, 2009
- Naive CD4+ T Cell Frequency Varies for Different Epitopes and Predicts Repertoire Diversity and Response MagnitudeImmunity, 2007
- Points of control in inflammationNature, 2002