GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype
Open Access
- 2 January 2020
- Vol. 9 (1), 120
- https://doi.org/10.3390/cells9010120
Abstract
Macrophages are critical for regulation of inflammatory response during endotoxemia and septic shock. However, the mediators underlying their regulatory function remain obscure. Growth differentiation factor 3 (GDF3), a member of transforming growth factor beta (TGF-β) superfamily, has been implicated in inflammatory response. Nonetheless, the role of GDF3 in macrophage-regulated endotoxemia/sepsis is unknown. Here, we show that serum GDF3 levels in septic patients are elevated and strongly correlate with severity of sepsis and 28-day mortality. Interestingly, macrophages treated with recombinant GDF3 protein (rGDF3) exhibit greatly reduced production of pro-inflammatory cytokines, comparing to controls upon endotoxin challenge. Moreover, acute administration of rGDF3 to endotoxin-treated mice suppresses macrophage infiltration to the heart, attenuates systemic and cardiac inflammation with less pro-inflammatory macrophages (M1) and more anti-inflammatory macrophages (M2), as well as prolongs mouse survival. Mechanistically, GDF3 is able to activate Smad2/Smad3 phosphorylation, and consequently inhibits the expression of nod-like receptor protein-3 (NLRP3) in macrophages. Accordingly, blockade of Smad2/Smad3 phosphorylation with SB431542 significantly offsets rGDF3-mediated anti-inflammatory effects. Taken together, this study uncovers that GDF3, as a novel sepsis-associated factor, may have a dual role in the pathophysiology of sepsis. Acute administration of rGDF3 into endotoxic shock mice could increase survival outcome and improve cardiac function through anti-inflammatory response by suppression of M1 macrophage phenotype. However, constitutive high levels of GDF3 in human sepsis patients are associated with lethality, suggesting that GDF3 may promote macrophage polarization toward M2 phenotype which could lead to immunosuppression.Funding Information
- National Institutes of Health (GM-126061, GM-132149)
- American Heart Association (17EIA33400063)
This publication has 41 references indexed in Scilit:
- Functional polarization of tumour-associated macrophages by tumour-derived lactic acidNature, 2014
- Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based ProteomicsMolecular & Cellular Proteomics, 2014
- Embryonic and Adult-Derived Resident Cardiac Macrophages Are Maintained through Distinct Mechanisms at Steady State and during InflammationImmunity, 2014
- TGFβ signaling plays a critical role in promoting alternative macrophage activationBMC Immunology, 2012
- Toward a better understanding of the interaction between TGF-β family members and their ALK receptorsJournal of Molecular Modeling, 2012
- Smad2 and Smad3 are redundantly essential for the suppression of iNOS synthesis in macrophages by regulating IRF3 and STAT1 pathwaysInternational Immunology, 2012
- Growth/differentiation factor 3 signals through ALK7 and regulates accumulation of adipose tissue and diet-induced obesityProceedings of the National Academy of Sciences of the United States of America, 2008
- Serum lactate as a predictor of mortality in patients with infectionIntensive Care Medicine, 2007
- The Vg1-related protein Gdf3 acts in a Nodal signaling pathway in the pre-gastrulation mouse embryoDevelopment, 2006
- SPLENECTOMY AND SEPSIS: THE ROLE OF THE SPLEEN IN THE IMMUNE-MEDIATED BACTERIAL CLEARANCEImmunopharmacology and Immunotoxicology, 2001