The Aqueous Extract of Polypodium leucotomos (Fernblock®) Regulates Opsin 3 and Prevents Photooxidation of Melanin Precursors on Skin Cells Exposed to Blue Light Emitted from Digital Devices
Open Access
- 6 March 2021
- journal article
- research article
- Published by MDPI AG in Antioxidants
- Vol. 10 (3), 400
- https://doi.org/10.3390/antiox10030400
Abstract
The effects of sun exposure on the skin and specifically those related to pigmentation disorders are well known. It has recently been shown that blue light leads to the induction of oxidative stress and long-lasting pigmentation. The protective effect of an aqueous extract of Polypodium leucotomos (Fernblock®) is known. Our aim was to investigate the action mechanism of Fernblock® against pigmentation induced by blue light from digital devices. Human fibroblasts (HDF) and murine melanocytes (B16-F10) were exposed to artificial blue light (a 400–500 nm LED lamp). Cell viability, mitochondrial morphology, and the expression of the mitogen-activated protein kinase (MAPK) p38, known markers involved in the melanogenesis pathway, were evaluated. The activation of Opsin-3, a membrane protein sensitive to blue light that triggers the activation of the enzyme tyrosinase responsible for melanogenesis in melanocytes, was also analyzed. Our results demonstrated that pretreatment with Fernblock® prevents cell death, alteration of mitochondrial morphology, and phosphorylation of p38 in HDF exposed to blue light. In addition, Fernblock® significantly reduced the activation of Opsin-3 in melanocytes and the photo-oxidation of melanin, preventing its photodegradation. In sum, Fernblock® exerts beneficial effects against the detrimental impact of blue light from digital devices and could prevent early photoaging, while maintaining skin homeostasis.Funding Information
- Comunidad Autonoma de Madrid (PEJ-2019-AI/BMD-12585)
This publication has 33 references indexed in Scilit:
- Irradiation of Skin with Visible Light Induces Reactive Oxygen Species and Matrix-Degrading EnzymesJournal of Investigative Dermatology, 2012
- Aging Skin: Histology, Physiology, and PathologyFacial Plastic Surgery Clinics of North America, 2011
- Blue-Light Irradiation Regulates Proliferation and Differentiation in Human Skin CellsJournal of Investigative Dermatology, 2010
- GSK3β inhibition promotes melanogenesis in mouse B16 melanoma cells and normal human melanocytesCellular Signalling, 2008
- The Protective Role of Melanin Against UV Damage in Human Skin†Photochemistry and Photobiology, 2007
- A Polypodium leucotomos extract inhibits solar‐simulated radiation‐induced TNF‐α and iNOS expression, transcriptional activation and apoptosisExperimental Dermatology, 2007
- Mechanism of the melanogenesis stimulation activity of (−)-cubebin in murine B16 melanoma cellsBioorganic & Medicinal Chemistry, 2007
- Dopaquinone redox exchange with dihydroxyindole and dihydroxyindole carboxylic acidPigment Cell Research, 2006
- Phenolic components and antioxidant activity of Fernblock, an aqueous extract of the aerial parts of the fern Polypodium leucotomosMethods and Findings in Experimental and Clinical Pharmacology, 2006
- Involvement of Photooxidation of Melanogenic Precursors in Prolonged Pigmentation Induced by Ultraviolet AJournal of Investigative Dermatology, 2004