Chronic Alcohol Exposure of Cells Using Controlled Alcohol-Releasing Capillaries
Open Access
- 6 May 2021
- Vol. 10 (5), 1120
- https://doi.org/10.3390/cells10051120
Abstract
Alcohol is one of the main causes of liver diseases such as fatty liver, alcoholic hepatitis, and chronic hepatitis with liver fibrosis or cirrhosis. To reproduce the conditions of alcohol-induced liver diseases and to identify the disease-causing mechanisms at the cellular level, several methods have been used to expose the cells to ethanol. As ethanol evaporates easily, it is difficult to mimic chronic alcohol exposure conditions at the cellular level. In this study, we developed a glass capillary system containing ethanol, which could steadily release ethanol from the polyethylene tubing and hydrogel portion at both sides of the capillary. The ethanol-containing capillary could release ethanol in the cell culture medium for up to 144 h, and the concentration of ethanol in the cell culture medium could be adjusted by controlling the number of capillaries. A long-term exposure to ethanol by the capillary system led to an increased toxicity of cells and altered the cellular physiologies, such as increasing the lipid accumulation and hepatic transaminase release in cells, as compared to the traditional direct ethanol addition method. Ethanol capillaries showed different gene expression patterns of lipid accumulation- or chronic alcoholism-related genes. Our results suggest that our ethanol-containing capillary system can be used as a valuable tool for studying the mechanism of chronic alcohol-mediated hepatic diseases at the cellular level.Funding Information
- National Research Foundation of Korea (2018R1A5A2025272)
This publication has 49 references indexed in Scilit:
- An in vitro model for studying the effects of continuous ethanol exposure on N-methyl-d-aspartate receptor functionAlcohol, 2012
- Radiological Imaging Features of Fasciola hepatica Infection – A Pictorial ReviewJournal of Clinical Imaging Science, 2012
- Acute Alcohol-Induced Liver InjuryFrontiers in Physiology, 2012
- Chronic Alcohol Exposure Alters Gene Expression in HepG2 CellsAlcohol: Clinical and Experimental Research, 2011
- An essential role for monocyte chemoattractant protein-1 in alcoholic liver injury: Regulation of proinflammatory cytokines and hepatic steatosis in miceJournal of Hepatology, 2011
- Huh-7 or HepG2 cells: which is the better model for studying human apolipoprotein-B100 assembly and secretion?Journal of Lipid Research, 2011
- Mechanical and cell viability properties of crosslinked low‐ and high‐molecular weight poly(ethylene glycol) diacrylate blendsJournal of Biomedical Materials Research Part A, 2009
- Inhibition of apoB secretion from HepG2 cells by insulin is amplified by naringenin, independent of the insulin receptorJournal of Lipid Research, 2008
- Detection of circulating antibodies against malondialdehyde-acetaldehyde adducts in patients with alcohol-induced liver diseaseJournal of Hepatology, 2000
- In Vitro Assessment of the Ethanol-Induced Hepatotoxicity on HepG2 Cell LineBiochemical and Biophysical Research Communications, 1993