The Scattered Twelve Tribes of HEK293
Open Access
- 13 June 2018
- journal article
- Published by Oriental Scientific Publishing Company in Biomedical and Pharmacology Journal
- Vol. 11 (2), 621-623
- https://doi.org/10.13005/bpj/1414
Abstract
Their ease of growth and transfection makes HEK293 cells a common cell culture in academic research. In addition, high transfection efficiency of HEK293 cells enable production of exogenous proteins or viruses for pharmaceutical and biomedical research purposes. Recently, HEK293 cells has gained attention due to it is versatility for transfection experiments, particularly the propagation of adenoviral-based and retroviral-based vectors during CART-T bioprocess. Since traceability is critical to pharmaceutical manufacturing process, we provide a mini review to clarify the historical development and intent use of different variants of HEK293 cells. This review should provide a key reference for the HEK293 variants’ historical and developmental background.Keywords
This publication has 17 references indexed in Scilit:
- Driving CAR T-cells forwardNature Reviews Clinical Oncology, 2016
- Influence of HEK293 metabolism on the production of viral vectors and vaccineVaccine, 2015
- HEK293 in cell biology and cancer research: phenotype, karyotype, tumorigenicity, and stress-induced genome-phenotype evolutionGene, 2015
- Engineering CAR-T cells: Design conceptsTrends in Immunology, 2015
- Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulationsNature Communications, 2014
- High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cellsBMC Biotechnology, 2013
- Expressing Full-Length Functional PfEMP1 Proteins in the HEK293 Expression SystemMethods in molecular biology (Clifton, N.J.), 2012
- HEK293 cell line: A vehicle for the expression of recombinant proteinsJournal of Pharmacological and Toxicological Methods, 2005
- Heat-evoked Activation of TRPV4 Channels in a HEK293 Cell Expression System and in Native Mouse Aorta Endothelial CellsOnline Journal of Public Health Informatics, 2002
- Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cellsThe FASEB Journal, 2002