Rapid Manufacturing of Multilayered Microfluidic Devices for Organ on a Chip Applications
Open Access
- 16 February 2021
- Vol. 21 (4), 1382
- https://doi.org/10.3390/s21041382
Abstract
Microfabrication and Polydimethylsiloxane (PDMS) soft-lithography techniques became popular for microfluidic prototyping at the lab, but even after protocol optimization, fabrication is yet a long, laborious process and partly user-dependent. Furthermore, the time and money required for the master fabrication process, necessary at any design upgrade, is still elevated. Digital Manufacturing (DM) and Rapid-Prototyping (RP) for microfluidics applications arise as a solution to this and other limitations of photo and soft-lithography fabrication techniques. Particularly for this paper, we will focus on the use of subtractive DM techniques for Organ-on-a-Chip (OoC) applications. Main available thermoplastics for microfluidics are suggested as material choices for device fabrication. The aim of this review is to explore DM and RP technologies for fabrication of an OoC with an embedded membrane after the evaluation of the main limitations of PDMS soft-lithography strategy. Different material options are also reviewed, as well as various bonding strategies. Finally, a new functional OoC device is showed, defining protocols for its fabrication in Cyclic Olefin Polymer (COP) using two different RP technologies. Different cells are seeded in both sides of the membrane as a proof of concept to test the optical and fluidic properties of the device.Keywords
Funding Information
- Commission for Universities and Research of the Department of Innovation, Universities, and Enterprise of the Generalitat de Catalunya (2017 SGR 1079, AdvanceCat and Base3D, TEC2015-70104-P, CTQ2016-75870-P)
- Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER Actions)
This publication has 45 references indexed in Scilit:
- Microfluidic Cell Culture and Its Application in High-Throughput Drug Screening: Cardiotoxicity Assay for hERG ChannelsSLAS Discovery, 2010
- Transport in two-dimensional paper networksMicrofluidics and Nanofluidics, 2010
- Cyclic olefin polymers: emerging materials for lab-on-a-chip applicationsMicrofluidics and Nanofluidics, 2010
- CYP1B1 and endothelial nitric oxide synthase combine to sustain proangiogenic functions of endothelial cells under hyperoxic stressAmerican Journal of Physiology-Cell Physiology, 2010
- Hyperoxia augments ER-stress-induced cell death independent of BiP lossFree Radical Biology & Medicine, 2009
- Biological implications of polydimethylsiloxane-based microfluidic cell cultureLab on a Chip, 2009
- Evaporation from microreservoirsLab on a Chip, 2009
- Microinjection molded disposable microfluidic lab-on-a-chip for efficient detection of agglutinationMicrosystem Technologies, 2008
- Managing evaporation for more robust microscale assays : Part 2. Characterization of convection and diffusion for cell biologyLab on a Chip, 2008
- Managing evaporation for more robust microscale assays : Part 1. Volume loss in high throughput assaysLab on a Chip, 2008