Chromosomal composition analysis and molecular marker development for the novel Ug99-resistant wheat–Thinopyrum ponticum translocation line WTT34
- 6 March 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 134 (5), 1587-1599
- https://doi.org/10.1007/s00122-021-03796-0
Abstract
Key message A novel Ug99-resistant wheat–Thinopyrum ponticum translocation line was produced, its chromosomal composition was analyzed and specific markers were developed. Abstract Stem rust caused by Puccinia graminis f. sp. tritici Eriks. & E. Henn (Pgt) has seriously threatened global wheat production since Ug99 race TTKSK was first detected in Uganda in 1998. Thinopyrum ponticum is near immune to Ug99 races and may be useful for enhancing wheat disease resistance. Therefore, developing new wheat–Th. ponticum translocation lines that are resistant to Ug99 is crucial. In this study, a novel wheat–Th. ponticum translocation line, WTT34, was produced. Seedling and field evaluation revealed that WTT34 is resistant to Ug99 race PTKST. The resistance was derived from the alien parent Th. ponticum. Screening WTT34 with markers linked to Sr24, Sr25, Sr26, Sr43, and SrB resulted in the amplification of different DNA fragments from Th. ponticum, implying WTT34 carries at least one novel stem rust resistance gene. Genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), and multi-color GISH (mc-GISH) analyses indicated that WTT34 carries a T5DS·5DL-Th translocation, which was consistent with wheat660K single-nucleotide polymorphism (SNP) array results. The SNP array also uncovered a deletion event in the terminal region of chromosome 1D. Additionally, the homeology between alien segments and the wheat chromosomes 2A and 5D was confirmed. Furthermore, 51 PCR-based markers derived from the alien segments of WTT34 were developed based on specific-locus amplified fragment sequencing (SLAF-seq). These markers may enable wheat breeders to rapidly trace Th. ponticum chromosomal segments carrying Ug99 resistance gene(s).Keywords
Funding Information
- National Key Research and Development Program of China (2016YFD0102000)
- National Natural Science Foundation of China (No. 31971875)
This publication has 55 references indexed in Scilit:
- The Development of 7E Chromosome-Specific Molecular Markers for Thinopyrum elongatum Based on SLAF-seq TechnologyPLOS ONE, 2013
- Construction of a high-density genetic map for sesame based on large scale marker development by specific length amplified fragment (SLAF) sequencingBMC Plant Biology, 2013
- Characterization of two new Puccinia graminis f. sp. tritici races within the Ug99 lineage in South AfricaEuphytica, 2010
- Circos: An information aesthetic for comparative genomicsGenome Research, 2009
- Development and Characterization of Expressed Sequence Tag-Derived Microsatellite Markers for the Wheat Stem Rust Fungus Puccinia graminis f. sp. triticiPhytopathology®, 2009
- Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum × Thinopyrum ponticum by in situ hybridization, isozyme analysis, and RAPDGenome, 1996
- Compensation Indices of Radiation‐Induced Wheat‐Agropyron elongatum Translocations Conferring Resistance to Leaf Rust and Stem RustCrop Science, 1994
- Cytogenetics of decaploid Agropyron elongatum (Elytrigia elongata) (2n = 70). I. Frequency of decavalent formationGenome, 1990
- Inheritance of leaf rust and stem rust resistances in wheat cultivars Agent and AgathaAustralian Journal of Agricultural Research, 1977
- Registration of Agent Wheat1 (Reg. No. 471)Crop Science, 1968