New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
Open Access
- 1 January 2020
- journal article
- research article
- Published by FapUNIFESP (SciELO) in Revista da Sociedade Brasileira de Medicina Tropical
- Vol. 53, e20190333
- https://doi.org/10.1590/0037-8682-0333-2019
Abstract
Introduction: Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species. Methods: We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification. Results: We proposed the use of the rpoC gene, encoding the DNA-directed RNA polymerase beta-subunit, for identifying 35 Leptospira species. Conclusions: The rpoC gene can be a molecular target to identify the main species of the Leptospira genus directly from clinical samples.Keywords
This publication has 14 references indexed in Scilit:
- Biodiversity of Environmental Leptospira: Improving Identification and Revisiting the DiagnosisFrontiers in Microbiology, 2018
- MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger DatasetsMolecular Biology and Evolution, 2016
- What Makes a Bacterial Species Pathogenic?:Comparative Genomic Analysis of the Genus LeptospiraPLoS Neglected Tropical Diseases, 2016
- Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNAPLOS ONE, 2015
- OrthoVenn: a web server for genome wide comparison and annotation of orthologous clusters across multiple speciesNucleic Acids Research, 2015
- Leptospira and leptospirosisVeterinary Microbiology, 2010
- InParanoid 7: new algorithms and tools for eukaryotic orthology analysisNucleic Acids Research, 2009
- The RAST Server: Rapid Annotations using Subsystems TechnologyBMC Genomics, 2008
- Species-Specific Identification of Leptospiraceae by 16S rRNA Gene SequencingJournal of Clinical Microbiology, 2006
- OrthoMCL: Identification of Ortholog Groups for Eukaryotic GenomesGenome Research, 2003