Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors
Open Access
- 2 December 2019
- journal article
- research article
- Published by Springer Science and Business Media LLC in Communications Biology
- Vol. 2 (1), 1-12
- https://doi.org/10.1038/s42003-019-0687-9
Abstract
Phytochrome photoreceptors mediate adaptive responses of plants to red and far-red light. These responses generally entail light-regulated association between phytochromes and other proteins, among them the phytochrome-interacting factors (PIF). The interaction with Arabidopsis thaliana phytochrome B (AtPhyB) localizes to the bipartite APB motif of the A. thaliana PIFs (AtPIF). To address a dearth of quantitative interaction data, we construct and analyze numerous AtPIF3/6 variants. Red-light-activated binding is predominantly mediated by the APB N-terminus, whereas the C-terminus modulates binding and underlies the differential affinity of AtPIF3 and AtPIF6. We identify AtPIF variants of reduced size, monomeric or homodimeric state, and with AtPhyB affinities between 10 and 700 nM. Optogenetically deployed in mammalian cells, the AtPIF variants drive light-regulated gene expression and membrane recruitment, in certain cases reducing basal activity and enhancing regulatory response. Moreover, our results provide hitherto unavailable quantitative insight into the AtPhyB:AtPIF interaction underpinning vital light-dependent responses in plants.This publication has 76 references indexed in Scilit:
- Multi-chromatic control of mammalian gene expression and signalingNucleic Acids Research, 2013
- A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cellsNucleic Acids Research, 2013
- Light-based feedback for controlling intracellular signaling dynamicsNature Methods, 2011
- A Brief History of PhytochromesChemphyschem, 2010
- Biological Insights from Structures of Two-Component ProteinsAnnual Review of Microbiology, 2009
- Spatiotemporal control of cell signalling using a light-switchable protein interactionNature, 2009
- Residues Clustered in the Light-Sensing Knot of Phytochrome B are Necessary for Conformer-Specific Binding to Signaling Partner PIF3PLoS Genetics, 2009
- TheArabidopsisPhytochrome-Interacting Factor PIF7, Together with PIF3 and PIF4, Regulates Responses to Prolonged Red Light by Modulating phyB LevelsTHE PLANT CELL ONLINE, 2008
- PHYTOCHROME STRUCTURE AND SIGNALING MECHANISMSAnnual Review of Plant Biology, 2006
- A guide to choosing fluorescent proteinsNature Methods, 2005