Hyperthermia depletes Oct4 in mouse blastocysts and stem cells
Open Access
- 24 May 2020
- journal article
- letter
- Published by Springer Science and Business Media LLC in Stem Cell Research & Therapy
- Vol. 11 (1), 1-6
- https://doi.org/10.1186/s13287-020-01715-6
Abstract
Temperature is an important microenvironmental factor that functions epigenetically in normal embryonic development. However, the effect of hyperthermia in the stem cells is not fully understood. Oct4 is a tightly regulated master regulator of pluripotency maintenance in stem cells and during early embryonic development. We report here that Oct4 protein level was significantly reduced under hyperthermia in mouse blastocysts and embryonic stem cells. The reduction in Oct4 in the mouse embryonic stem cells under hyperthermia was mediated by a ubiquitin-proteasome pathway that was dependent on the activity of death-associated protein kinase 1 (Dapk1) to phosphorylate its substrate, Pin1. Our results imply that the depletion of Oct4 via brief hyperthermia, such as a high fever, during early pregnancy might severely impair the growth of the mammalian embryo or even cause its death.Keywords
Funding Information
- Chinese Academy of Medical Sciences (2016-I2M-3-002, 2017-I2M-3-009)
- National Natural Science Foundation of China (91519301, 31871310)
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