Harnessing the Periplasm of Bacterial Cells To Develop Biocatalysts for the Biosynthesis of Highly Pure Chemicals
- 1 January 2018
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 84 (1), e01693-17
- https://doi.org/10.1128/aem.01693-17
Abstract
Although biocatalytic transformation has shown great promise in chemical synthesis, there remain significant challenges in controlling high selectivity without the formation of undesirable by-products. For instance, few attempts to construct biocatalysts for de novo synthesis of pure flavin mononucleotide (FMN) have been successful, due to riboflavin (RF) accumulating in the cytoplasm and being secreted with FMN. To address this problem, we show here a novel biosynthesis strategy, compartmentalizing the final FMN biosynthesis step in the periplasm of an engineered Escherichia coli strain. This construct is able to overproduce FMN with high specificity (92.4% of total excreted flavins). Such a biosynthesis approach allows isolation of the final biosynthesis step from the cytoplasm to eliminate undesirable by-products, providing a new route to develop biocatalysts for the synthesis of high-purity chemicals.Keywords
Funding Information
- China Postdoctoral Science Foundation (2016M591043)
- Ministry of Education - Singapore (AcRF Tier 1 (M4011622.030))
This publication has 39 references indexed in Scilit:
- New and improved tools and methods for enhanced biosynthesis of natural products in microorganismsCurrent Opinion in Biotechnology, 2016
- Metabolic engineering of Escherichia coli W3110 to produce L‐malateBiotechnology & Bioengineering, 2016
- Enhancing fatty acid production in Escherichia coli by Vitreoscilla hemoglobin overexpressionBiotechnology & Bioengineering, 2016
- Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integrationBiotechnology & Bioengineering, 2016
- Whole‐cell biocatalytic and de novo production of alkanes from free fatty acids in Saccharomyces cerevisiaeBiotechnology & Bioengineering, 2015
- Systems strategies for developing industrial microbial strainsNature Biotechnology, 2015
- The ‘PHAome’Trends in Biotechnology, 2015
- An ancient Chinese wisdom for metabolic engineering: Yin-YangMicrobial Cell Factories, 2015
- Synthesis of many different types of organic small molecules using one automated processScience, 2015
- Enantioselective trans-Dihydroxylation of Aryl Olefins by Cascade Biocatalysis with Recombinant Escherichia coli Coexpressing Monooxygenase and Epoxide HydrolaseACS Catalysis, 2014