Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair
Top Cited Papers
- 26 February 2021
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 371 (6532), 905-+
- https://doi.org/10.1126/science.abc4346
Abstract
Organ homeostasis is orchestrated by time- and spatially restricted cell proliferation. Studies identifying cells with superior proliferative capacities often rely on the lineage tracing of a subset of cell populations, which introduces a potential selective bias. In this work, we developed a genetic system [proliferation tracer (ProTracer)] by incorporating dual recombinases to seamlessly record the proliferation events of entire cell populations over time in multiple organs. In the mouse liver, ProTracer revealed more hepatocyte proliferation in distinct zones during liver homeostasis, injury repair, and regrowth. Clonal analysis showed that most of the hepatocytes labeled by ProTracer had undergone cell division. By genetically recording proliferation events of entire cell populations, ProTracer enables the unbiased detection of specific cellular compartments with enhanced regenerative capacities.Keywords
Funding Information
- China Postdoctoral Science Foundation
- National Key Research and Development Program of China Stem Cell and Translational Research (2019YFA0110403, 2018YFA0107900, 2019YFA0802000)
- National Key Research and Development Program of China Stem Cell and Translational Research (2018YFA0108100, 2017YFC1001303)
- National Science Foundation of China (31730112, 31625019, 91849202, 81761138040)
- National Science Foundation of China (81872241, 31922032)
- the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB19000000, XDA16010507)
- Shanghai Rising Star Project
- Innovative research team of high-level local universities in Shanghai, Shanghai Science and Technology Commission (19ZR1479800)
- Royal Society-Newton Advanced Fellowship
- Youth Innovation Promotion Association of CAS, Key Project of Frontier Sciences of CAS (QYZDB-SSW-SMC003)
- Innovative research team of high-level local universities in Shanghai, Shanghai Science and Technology Commission (17ZR1449600)
- the Program for Guangdong Introduction Innovative and Entrepreneurial Teams (2017ZT07S347)
- China Postdoctoral Innovative Talent Support Program
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