Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis
Open Access
- 13 January 2021
- journal article
- research article
- Published by Wiley in Journal of Cellular and Molecular Medicine
- Vol. 25 (4), 2238-2253
- https://doi.org/10.1111/jcmm.16217
Abstract
The peritoneum, especially the omentum, is a common site for gastric cancer (GC) metastasis. Our aim was to expound the role and mechanisms of Piezo1 on GC omentum metastasis. A series of functional assays were performed to examine cell proliferation, clone formation, apoptosis, Ca2+ influx, mitochondrial membrane potential (MMP) and migration after overexpression or knockdown of Piezo1. A GC peritoneal implantation and metastasis model was conducted. After infection by si‐Piezo1, the number and growth of tumours were observed in abdominal cavity. Fibre and angiogenesis were tested in metastatic tumour tissues. Piezo1 had higher expression in GC tissues with omentum metastasis and metastatic lymph node tissues than in GC tissues among 110 patients. High Piezo1 expression was associated with lymph metastasis, TNM and distant metastasis. Overexpressed Piezo1 facilitated cell proliferation and suppressed cell apoptosis in GC cells. Moreover, Ca2+ influx was elevated after up‐regulation of Piezo1. Piezo1 promoted cell migration and Calpain1/2 expression via up‐regulation of HIF‐1α in GC cells. In vivo, Piezo1 knockdown significantly inhibited peritoneal metastasis of GC cells and blocked EMT process and angiogenesis. Our findings suggested that Piezo1 is a key component during GC omentum metastasis, which could be related to up‐regulation of HIF‐1α.Keywords
This publication has 31 references indexed in Scilit:
- Inhibition of peritoneal metastasis of human gastric cancer cells by dextran sulphate through the reduction in HIF-1α and ITGβ1 expressionOncology Reports, 2016
- Ion Permeation and Mechanotransduction Mechanisms of Mechanosensitive Piezo ChannelsNeuron, 2016
- NNC 55-0396, a T-type Ca2+ channel inhibitor, inhibits angiogenesis via suppression of hypoxia-inducible factor-1α signal transductionJournal of Molecular Medicine, 2014
- Coexpression of CD44‐positive/CD133‐positive cancer stem cells and CD204‐positive tumor‐associated macrophages is a predictor of survival in pancreatic ductal adenocarcinomaCancer, 2014
- Piezo1 Is as a Novel Trefoil Factor Family 1 Binding Protein that Promotes Gastric Cancer Cell Mobility In VitroDigestive Diseases and Sciences, 2014
- Intraperitoneal chemotherapy in advanced gastric cancer. Meta-analysis of randomized trialsEuropean Journal of Surgical Oncology, 2014
- Mitochondria and cancerNature Reviews Cancer, 2012
- Piezo proteins are pore-forming subunits of mechanically activated channelsNature, 2012
- Myometrial Invasion and Lymph Node Metastasis in Endometrioid Carcinomas: Tumor-associated Macrophages, Microvessel Density, and HIF1A Have a Crucial RoleThe American Journal of Surgical Pathology, 2010
- Targeting mitochondria for cancer therapyNature Reviews Drug Discovery, 2010