Global phenotypic profiling identifies a conserved actinobacterial cofactor for a bifunctional PBP-type cell wall synthase
Open Access
- 13 March 2020
- journal article
- research article
- Published by eLife Sciences Publications, Ltd in eLife
Abstract
Members of the Corynebacterineae suborder of Actinobacteria have a unique cell surface architecture and, unlike most well-studied bacteria, grow by tip-extension. To investigate the distinct morphogenic mechanisms shared by these organisms, we performed a genome-wide phenotypic profiling analysis using Corynebacterium glutamicum as a model. A high-density transposon mutagenized library was challenged with a panel of antibiotics and other stresses. The fitness of mutants in each gene under each condition was then assessed by transposon-sequencing. Clustering of the resulting phenotypic fingerprints revealed a role for several genes of previously unknown function in surface biogenesis. Further analysis identified CofA (Cgp_0016) as an interaction partner of the peptidoglycan synthase PBP1a that promotes its stable accumulation at sites of polar growth. The related Mycobacterium tuberculosis proteins were also found to interact, highlighting the utility of our dataset for uncovering conserved principles of morphogenesis for this clinically relevant bacterial suborder.Funding Information
- National Institute of Allergy and Infectious Diseases (AI083365)
- National Institute of Allergy and Infectious Diseases (AI132120)
- Life Sciences Research Foundation
- Howard Hughes Medical Institute (Faculty Scholar Award)
- Simons Foundation (Faculty Scholar Award)
- Howard Hughes Medical Institute (Investigator funds)
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