Double mutation of two homologous genes YL1 and YL2 results in a leaf yellowing phenotype in soybean [Glycine max (L.) Merr]
- 1 July 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Plant Molecular Biology
- Vol. 103 (4-5), 527-543
- https://doi.org/10.1007/s11103-020-01008-9
Abstract
Key message Two homologous, chloroplast located CAAX proteases were identified to be functional redundancy in determining soybean leaf color, and they probably play essential roles in regulating light harvesting and absorption during photosynthesis process. Leaf color mutants are ideal materials for studying photosynthesis and chlorophyll metabolism. The soybean [Glycine max (L.) Merr.] yellowing leaf (yl) variation is a recombinant mutant characterized by yellow foliage, which derived from the specific cross between green seed-coated and yellow seed-coated soybean varieties. Molecular cloning and subsequent gene silencing revealed that the yellow leaf trait of yl was controlled by two recessive nuclear genes, glyma11g04660 and glyma01g40650, named as YL1 and YL2 respectively, and the latter was confirmed to be same as the earlier reported green seed-coat gene G. Both YL1 and YL2 belonged to chloroplast-located proteases possessing Abi domain, and these genes were expressed in various tissues, especially in young leaves. In yl, the expression of YL1 and YL2 were suppressed in most tissues, and the young leaf of yl presented an increased maximal photochemical efficiency (Fv/Fm) as well as enhanced net photosynthesis activity (Pn), indicating that YL1 and YL2 are involved in light absorption regulation during photosynthesis process. Collectively, the identification and description of YL1 and YL2 in our study provides insights for the regulatory mechanism of photosynthesis process, and these findings will further assist to clarify the close relationship between photosynthesis and chlorophyll metabolism.Keywords
Funding Information
- The National Key R & D Program of China (2016YFD0100201)
- the National Transgenic Major Program of China (2016ZX08004001)
- The National Natural Science Foundation of China (31271750, 31571691, 31871646)
- the MOE Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT_17R55)
- the Fundamental Research Funds for the Central Universities (KYT201801)
- the Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP)
This publication has 39 references indexed in Scilit:
- Artificial selection for determinate growth habit in soybeanProceedings of the National Academy of Sciences, 2010
- Functional Analysis of Arabidopsis Postprenylation CaaX Processing Enzymes and Their Function in Subcellular Protein TargetingPlant Physiology, 2008
- MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequencesBriefings in Bioinformatics, 2008
- Validation of internal control for gene expression study in soybean by quantitative real-time PCRBMC Molecular Biology, 2008
- Gene duplication and paleopolyploidy in soybean and the implications for whole genome sequencingBMC Genomics, 2007
- Rice NON-YELLOW COLORING1 Is Involved in Light-Harvesting Complex II and Grana Degradation during Leaf SenescencePlant Cell, 2007
- Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.Proceedings of the National Academy of Sciences, 1991
- MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populationsGenomics, 1987
- Genetic Control of Chloroplast Pigment Development in Soybeans as a Function of Leaf and Plant MaturityPlant Physiology, 1981
- ON THE INHERITANCE OF CHLOROPHYLL COLORATIONS OF COTYLEDONS AND SEEDCOATS IN THE SOY BEANThe Japanese Journal of Genetics, 1929