In vivo T1 mapping for quantifying glymphatic system transport and cervical lymph node drainage
Open Access
- 3 September 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 10 (1), 1-13
- https://doi.org/10.1038/s41598-020-71582-x
Abstract
Dynamic contrast-enhanced magnetic resonance imaging (MRI) for tracking glymphatic system transport with paramagnetic contrast such as gadoteric acid (Gd-DOTA) administration into cerebrospinal fluid (CSF) requires pre-contrast data for proper quantification. Here we introduce an alternative approach for glymphatic system quantification in the mouse brain via T1 mapping which also captures drainage of Gd-DOTA to the cervical lymph nodes. The Gd-DOTA injection into CSF was performed on the bench after which the mice underwent T1 mapping using a 3D spoiled gradient echo sequence on a 9.4 T MRI. In Ketamine/Xylazine (KX) anesthetized mice, glymphatic transport and drainage of Gd-DOTA to submandibular and deep cervical lymph nodes was demonstrated as 25–50% T1 reductions in comparison to control mice receiving CSF saline. To further validate the T1 mapping approach we also verified increased glymphatic transport of Gd-DOTA transport in mice anesthetized with KX in comparison with ISO. The novel T1 mapping method allows for quantification of glymphatic transport as well as drainage to the deep and superficial cervical lymph nodes. The ability to measure glymphatic transport and cervical lymph node drainage in the same animal longitudinally is advantageous and time efficient and the coupling between the two systems can be studied and translated to human studies.Keywords
Funding Information
- NIH (R01AG057705, R01AG057705, R01AG057705, R01AG057705, R01AG057705)
- Fondation Leducq (16/CVD/05, 16/CVD/05)
This publication has 45 references indexed in Scilit:
- Sleep Drives Metabolite Clearance from the Adult BrainScience, 2013
- Brain-wide pathway for waste clearance captured by contrast-enhanced MRIJCI Insight, 2013
- A Paravascular Pathway Facilitates CSF Flow Through the Brain Parenchyma and the Clearance of Interstitial Solutes, Including Amyloid βScience Translational Medicine, 2012
- Histological Study and LYVE-1 Immunolocalization of Mouse Mesenteric Lymph Nodes with "In Vivo Cryotechnique"ACTA HISTOCHEMICA ET CYTOCHEMICA, 2011
- In vivo 3D digital atlas database of the adult C57BL/6J mouse brain by magnetic resonance microscopyFrontiers in Neuroanatomy, 2008
- T1 relaxation in in vivo mouse brain at ultra‐high fieldMagnetic Resonance in Medicine, 2007
- High magnetic field water and metabolite proton T1 and T2 relaxation in rat brain in vivoMagnetic Resonance in Medicine, 2006
- Anatomy and nomenclature of murine lymph nodes: Descriptive study and nomenclatory standardization in BALB/cAnNCrl miceJournal of Immunological Methods, 2006
- Unified segmentationNeuroImage, 2005
- In vivo measurements of T1 relaxation times in mouse brain associated with different modes of systemic administration of manganese chlorideJournal of Magnetic Resonance Imaging, 2005