The Therapeutic Effects of Magnolia Officinalis Extraction on an Antibiotics-Induced Intestinal Dysbacteriosis in Mice
- 19 June 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Current Microbiology
- Vol. 77 (9), 2413-2421
- https://doi.org/10.1007/s00284-020-02056-6
Abstract
Magnolia officinalis bark is a traditional Chinese medicine for gastrointestinal tract disorders. In this study, we explored the effects of M. officinalis extraction on intestinal flora to reveal its mechanism. Thirty SPF mice were divided into five groups: C (control), M (M. officinalis), A (antibiotics: cefradine and gentamicin sulfate), A&M (antibiotics + M. officinalis) and A&N (antibiotics + natural recovery). Faecal samples of all groups were collected and the taxonomic composition and diversity of bacteria was characterized using the 16S rRNA gene (16S). Alpha diversity showed gut bacteria diversity significantly decreased in the A group of mice but increased markedly after administration of M. officinalis extract. Beta diversity indicated that C, M and A&M shared similar bacterial community structure while A and A&N exhibited a different bacterial community. Furthermore, RDA combined with spearman correlation heatmap suggested the five physiological indicators (weight, fur, activity and feces) were highly correlated with bacterial community structure and diversity. Finally, functional categorization of the assigned OTUs was performed using the PICRUSt tool. The changes in PICRUSt inferred that function profile and metabolic pathways were observed in A and A&M, therefore the M. officinalis extract improved the intestinal flora of A&M and normalized its metabolic pathways gradually, improving mouse weight, fur quality, activity and feces qualities.Keywords
Funding Information
- National Natural Science Foundation of China (No.81202933)
- Department of Science and Technology of Sichuan Province (2013szz025)
- Sichuan Province Department of Education Science-technology Plan Projects (No.18ZA0267)
- Mianyang Science and Technology Plan Projects (2018YFZJ025)
- Mianyang Normal University Science-technology Plan Projects (No. QD2014A006)
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