Developing and population analysis of a new multiplex panel of 18 microhaplotypes and compound markers using next generation sequencing and its application in the Shaanxi Han population
- 24 April 2020
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 41 (13-14), 1230-1237
- https://doi.org/10.1002/elps.201900451
Abstract
Compound marker consists of two different types of genetic markers like deletion/insertion polymorphism (DIP) and single nucleotide polymorphism (SNP) in the genomic region of 200 bp, and microhaplotype consists of a series of closely linked SNPs in a small DNA segment (< 300 bp), which show great potential for human identifications and mixture analyses. In this study, we initially selected 23 novel genetic markers comprising 10 microhaplotypes and 13 compound markers according to previously reported SNP or DIP loci. Genetic distributions of these 23 loci in different continental populations showed that they could be used as valuable loci for forensic human identification purpose. Besides, high informativeness values (>0.1) were observed in six loci which could be further employed for forensic ancestry analyses. Finally, 18 loci were successfully developed into a multiplex panel and detected by the next generation sequencing (NGS) technology. Further analyses of the 18 loci in the studied Shaanxi Han population showed that 15 loci exhibited relatively high expected heterozygosities (>0.5). Cumulative power of discrimination (0.999 999 999 99 4835) of the 18 loci revealed that the multiplex panel could also be utilized for human identifications in the studied Shaanxi Han population. This article is protected by copyright. All rights reservedKeywords
Funding Information
- National Natural Science Foundation of China (81525015)
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