Tender Coconut Water Protects Mice From Ischemia-Reperfusion-Mediated Liver Injury and Secondary Lung Injury
- 3 March 2021
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Shock
- Vol. 56 (5), 762-772
- https://doi.org/10.1097/shk.0000000000001770
Abstract
Organ Injury by oxidative and inflammatory mediators occurs during ischemia-reperfusion (I/R) of the liver. Remote organ injury secondary to liver I/R increases the systemic insult. Tender coconut water (TCW) has been studied in chemical and fructose induced liver injury but its ability to decrease tissue injury in clinically relevant injury models is unknown. We evaluated the therapeutic potential of TCW in preventing liver I/R injury and associated remote organ injury. Mice were fed sugar (SUG) control water or TCW for a week and then subjected to 60 min of liver ischemia followed by reperfusion for 6 h. Plasma ALT levels, tissue damage and mRNA levels of Nos2, Tnf and Il6 were significantly lower in mice fed TCW prior to I/R. Plasma cytokines followed liver cytokine patterns. TCW increased mRNA levels of the anti-oxidant genes Hmox1 and Ptgs2 in the liver of mice subjected to I/R. Remote lung injury from liver I/R was also decreased by TCW feeding as evident by less neutrophil infiltration, decreased pro-inflammatory Il6, and increased anti-inflammatory Il10 mRNA levels in the lung. To examine macrophage activation as a potential mechanism, TCW pretreatment decreased in the amount of nitrite produced by RAW264.7 macrophages stimulated with LPS. The levels of Nos2, Il1b, Tnf and Il6 were decreased while Il10 and Hmox1 mRNA levels were significantly up-regulated upon LPS stimulation of TCW pretreated RAW264.7 macrophages. Collectively, our results indicate that TCW decreased hepatic I/R mediated damage to liver and lung and suggest that decreased macrophage activation contributes to this effect.Keywords
This publication has 47 references indexed in Scilit:
- Tumor Necrosis Factor-α in Liver Ischemia/Reperfusion InjuryJournal of Investigative Surgery, 2011
- The Chemical Composition and Biological Properties of Coconut (Cocos nucifera L.) WaterMolecules, 2009
- Constituents and antioxidant activity of two varieties of coconut water (Cocos nucifera L.)Revista Brasileira de Farmacognosia, 2009
- Ferulic Acid: Therapeutic Potential Through Its Antioxidant PropertyJournal of Clinical Biochemistry and Nutrition, 2007
- HEPATIC ISCHEMIA/REPERFUSION UPREGULATES THE SUSCEPTIBILITY OF HEPATOCYTES TO CONFER THE INDUCTION OF INDUCIBLE NITRIC OXIDE SYNTHASE GENE EXPRESSIONShock, 2006
- Expression of Heme Oxygenase‐1 in Human Livers Before Transplantation Correlates with Graft Injury and Function After TransplantationAmerican Journal of Transplantation, 2005
- Prostaglandin E2 inhibits transforming growth factor β1-mediated induction of collagen α1(I) in hepatic stellate cellsJournal of Hepatology, 2004
- A Protective Role for Cyclooxygenase-2 in Drug-Induced Liver Injury in MiceChemical Research in Toxicology, 2001
- Interleukin-10 suppresses hepatic ischemia/reperfusion injury in mice: Implications of a central role for nuclear factor ?BHepatology, 1999
- Protection of rat hepatocytes from tert-butyl hydroperoxide-induced injury by catecholToxicology and Applied Pharmacology, 1986