Background Alopecia areata (AA) is a complex autoimmune condition with increase of some pro-inflammatory mediators. The association of the novel neurokinin A with AA is still unclear. Data concerning other inflammatory diseases suggest a possible role of this neurokinin A in the pathophysiology of AA Aim The aim of this work was to evaluate serum level of neurokinin A in patients with AA and the impact of the AA on dermatological life quality index (DLQI) and depression anxiety stress scale (DASS). Patients and Methods Neurokinin A concentrations were measured in 50 patients with AA and 30 healthy controls using enzyme-linked immunosorbent assay (ELISA). Results The mean serum levels of neurokinin A was higher in patients than controls with significance p value is (<0.001). It was higher in male patients than females with no significance (p= 0.668). It was statistically significant higher in patient with chronic disease (p=0.023). There was statistically significant positive correlation found between serum neurokinin A level and severity of alopecia tool (SALT) score and stress in DASS subscales (as, p= <0.001, p=0.025) respectively Conclusion The mean serum levels of neurokinin A was higher in patients than controls with significance p value. It was statistically significant higher in patient with chronic disease also There was statistically significant positive correlation between serum neurokinin A level and SALT score and stress in DASS subscales.