A novel isolated Terrabacter-like bacterium can mineralize 2-aminopyrimidine, the principal metabolite of microbial sulfadiazine degradation
- 26 February 2015
- journal article
- Published by Springer Science and Business Media LLC in Biodegradation
- Vol. 26 (2), 139-150
- https://doi.org/10.1007/s10532-015-9722-9
Abstract
Recently we showed that during the degradation of sulfadiazine (SDZ) by Microbacterium lacus strain SDZm4 the principal metabolite 2-aminopyrimidine (2-AP) accumulated to the same molar amount in the culture as SDZ disappeared (Tappe et al. Appl Environ Microbiol 79:2572–2577, 2013). Although 2-AP is considered a recalcitrant agent, long-term lysimeter experiments with 14C-pyrimidine labeled SDZ ([14C]pyrSDZ) provided indications for substantial degradation of the pyrimidine moiety of the SDZ molecule. Therefore, we aimed to enrich 2-AP degrading bacteria and isolated a pure culture of a Terrabacter-like bacterium, denoted strain 2APm3. When provided with 14C-labeled SDZ, M. lacus strain SDZm4 degraded [14C]pyrSDZ to [14C]2-AP. Resting cells of 2APm3 at a concentration of 5 × 106 cells ml−1 degraded 62 µM [14C]2-AP to below the detection limit (0.6 µM) within 5 days. Disappearance of 2-AP resulted in the production of at least two transformation products (M1 and M2) with M2 being identified as 2-amino-4-hydroxypyrimidine. After 36 days, the transformation products disappeared and 83 % of the applied [14C]2-AP radioactivity was trapped as 14CO2. From this we conclude that a consortium of two species should be able to almost completely degrade SDZ in soils.Keywords
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