This study shortens the laboratory service time by shortening the centrifuge time and obtaining serum as an examination material. The research method used was pre-experimental research. The results showed that the average LDH in the blood group centrifuged 3000 rpm 5 minutes was 309.9 U / L and 306.4 U / L centrifuged 4400 rpm 3 minutes in the blood group. In conclusion, there is no difference in serum LDH levels from blood centrifuged at 3000 rpm for 5 minutes and 4400 rpm for 3 minutes. Keywords: Centrifugation, Lactate Dehydrogenase, TAT