Syntrophic Hydrocarbon Degradation in a Decommissioned Off-Shore Subsea Oil Storage Structure
Open Access
- 11 February 2021
- journal article
- research article
- Published by MDPI AG in Microorganisms
- Vol. 9 (2), 356
- https://doi.org/10.3390/microorganisms9020356
Abstract
Over the last decade, metagenomic studies have revealed the impact of oil production on the microbial ecology of petroleum reservoirs. However, despite their fundamental roles in bioremediation of hydrocarbons, biocorrosion, biofouling and hydrogen sulfide production, oil field and oil production infrastructure microbiomes are poorly explored. Understanding of microbial activities within oil production facilities is therefore crucial for environmental risk mitigation, most notably during decommissioning. The analysis of the planktonic microbial community from the aqueous phase of a subsea oil-storage structure was conducted. This concrete structure was part of the production platform of the Brent oil field (North Sea), which is currently undergoing decommissioning. Quantification and sequencing of microbial 16S rRNA genes, metagenomic analysis and reconstruction of metagenome assembled genomes (MAGs) revealed a unique microbiome, strongly dominated by organisms related to Dethiosulfatibacter and Cloacimonadetes. Consistent with the hydrocarbon content in the aqueous phase of the structure, a strong potential for degradation of low molecular weight aromatic hydrocarbons was apparent in the microbial community. These degradation pathways were associated with taxonomically diverse microorganisms, including the predominant Dethiosulfatibacter and Cloacimonadetes lineages, expanding the list of potential hydrocarbon degraders. Genes associated with direct and indirect interspecies exchanges (multiheme type-C cytochromes, hydrogenases and formate/acetate metabolism) were widespread in the community, suggesting potential syntrophic hydrocarbon degradation processes in the system. Our results illustrate the importance of genomic data for informing decommissioning strategies in marine environments and reveal that hydrocarbon-degrading community composition and metabolisms in man-made marine structures might differ markedly from natural hydrocarbon-rich marine environments.Keywords
Funding Information
- Shell Global Solutions International (-)
This publication has 56 references indexed in Scilit:
- Metagenome, metatranscriptome and single-cell sequencing reveal microbial response to Deepwater Horizon oil spillThe ISME Journal, 2012
- SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell SequencingJournal of Computational Biology, 2012
- IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depthBioinformatics, 2012
- GeobacterAdvances in Microbial Physiology, 2011
- QIIME allows analysis of high-throughput community sequencing dataNature Methods, 2010
- Syntrophy in anaerobic global carbon cyclesCurrent Opinion in Biotechnology, 2009
- Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial CommunitiesApplied and Environmental Microbiology, 2009
- IMG ER: a system for microbial genome annotation expert review and curationBioinformatics, 2009
- KEGG for linking genomes to life and the environmentNucleic Acids Research, 2007
- SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARBNucleic Acids Research, 2007