Astragaloside IV ameliorates radiation-induced senescence via antioxidative mechanism
- 15 May 2020
- journal article
- research article
- Published byĀ Oxford University Press (OUP)Ā inĀ Journal of Pharmacy and Pharmacology
- Vol.Ā 72Ā (8), 1110-1118
- https://doi.org/10.1111/jphp.13284
Abstract
Objectives Ageing is a universal and gradual process of organ deterioration. Radiation induces oxidative stress in cells, which leads to genetic damage and affects cell growth, differentiation and senescence. Astragaloside (AS)āIV has antioxidative, antiāapoptotic and antiāinflammatory properties. Methods To study the protective mechanism of ASāIV on radiationāinduced brain cell senescence, we constructed a radiationāinduced brain cell ageing model, using biochemical indicators, senescenceāassociated galactosidase (SAāĪ²āgal) senescence staining, flow cytometry and Western blotting to analyse the ASāIV resistance mechanism to radiationāinduced brain cell senescence. Key findings Radiation reduced superoxide dismutase (SOD) activity and expressions of cyclinādependent kinase (CDK2), CDK4, cyclin E and transcription factor E2F1 proteins, and increased expressions of p21, p16, cyclin D and retinoblastoma (RB) proteins, malondialdehyde (MDA) activity, SAāĪ²āgalāpositive cells and cells stagnating in G1 phase. After treatment with ASāIV, the level of oxidative stress in cells significantly decreased and expression of proteins related to the cell cycle and ageing significantly changed. In addition, SAāĪ²āgalāpositive cells and cells arrested in G1 phase were significantly reduced. Conclusions These data suggest that ASāIV can antagonize radiationāinduced brain cells senescence; and its mechanism may be related to p53āp21 and p16āRB signalling pathways of ageing regulation.Keywords
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