Synthesis and Characterization of a Leucine-Based Block Co-Polypeptide: The Effect of the Leucine Zipper on Self-Assembly
- 7 May 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 21 (6), 2463-2472
- https://doi.org/10.1021/acs.biomac.0c00420
Abstract
The self-assembly behavior of an ABC triblock copolypeptide consisting of poly(ethylene oxide-b-(leucine-s-valine)-b-lysine) (PEO−PLV−PK) was examined via dynamic light scattering in dilute aqueous solution. Leucine is a hydrophobic, α-helix forming polypeptide that exhibits a “zipper effect” in coiled-coil dimers. We hypothesize that the specific interaction afforded by the leucine zipper dominates the thermodynamics of self-assembly through the side-by-side ordering of α-helices, which drives vesicle formation in a polymer with only 6 wt % hydrophobic content. Additionally, a multitude of assembly sizes and morphologies were attainable from a single polymer, depending on the solution processing method. Thermodynamic effects of the leucine zipper can be interpreted, in part, from solubility parameters determined from molecular modeling. The combination of synthesis, solvent processing, and computational studies helps to elucidate the thermodynamic effects of this unique assembly motif on classical self-assembly processes.Keywords
Funding Information
- Division of Chemistry (1539347)
- American Chemical Society
- Golden Corral Charitable Fund
This publication has 48 references indexed in Scilit:
- Probing Membrane Hydration at the Interface of Self-Assembled Peptide Amphiphiles Using Electron Paramagnetic ResonanceACS Macro Letters, 2018
- 50th Anniversary Perspective: Block Polymers—Pure PotentialMacromolecules, 2016
- Polypeptide vesicles with densely packed multilayer membranesSoft Matter, 2015
- Self-assembly of block copolymersChemical Society Reviews, 2012
- Self‐Assembled Block Copolymer Aggregates: From Micelles to Vesicles and their Biological ApplicationsMacromolecular Rapid Communications, 2009
- Stimuli-responsive polypeptide vesicles by conformation-specific assemblyNature Materials, 2004
- Vesicles with asymmetric membranes from amphiphilic ABC triblock copolymersChemical Communications, 2002
- Polymer VesiclesScience, 2002
- Multiple Morphologies of "Crew-Cut" Aggregates of Polystyrene- b -poly(acrylic acid) Block CopolymersScience, 1995
- Polystyrene-Dendrimer Amphiphilic Block Copolymers with a Generation-Dependent AggregationScience, 1995