Base editors for simultaneous introduction of C-to-T and A-to-G mutations
Top Cited Papers
- 1 June 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 38 (7), 865-869
- https://doi.org/10.1038/s41587-020-0509-0
Abstract
We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. On-target as well as DNA and RNA off-target activities of Target-ACEmax were similar to those of existing single-function base editors.Keywords
Funding Information
- Japan Agency for Medical Research and Development
- Yamagata Prefectural Government and Tsuruoka City
- MEXT | Japan Science and Technology Agency
- The Uehara Memorial FoundationThe NOVARTIS Foundation (Japan) for the Promotion of ScienceNaito FoundationThe SECOM Science and Technology Foundation
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