TaDrAp1 and TaDrAp2, Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat
Open Access
- 5 November 2020
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (21), 8296
- https://doi.org/10.3390/ijms21218296
Abstract
Down-regulator associated protein, DrAp1, acts as a negative cofactor (NC2α) in a transcription repressor complex together with another subunit, down-regulator Dr1 (NC2β). In binding to promotors and regulating the initiation of transcription of various genes, DrAp1 plays a key role in plant transition to flowering and ultimately in seed production. TaDrAp1 and TaDrAp2 genes were identified, and their expression and genetic polymorphism were studied using bioinformatics, qPCR analyses, a 40K Single nucleotide polymorphism (SNP) microarray, and Amplifluor-like SNP genotyping in cultivars of bread wheat (Triticum aestivum L.) and breeding lines developed from a cross between spelt (T. spelta L.) and bread wheat. TaDrAp1 was highly expressed under non-stressed conditions, and at flowering, TaDrAp1 expression was negatively correlated with yield capacity. TaDrAp2 showed a consistently low level of mRNA production. Drought caused changes in the expression of both TaDrAp1 and TaDrAp2 genes in opposite directions, effectively increasing expression in lower yielding cultivars. The microarray 40K SNP assay and Amplifluor-like SNP marker, revealed clear scores and allele discriminations for TaDrAp1 and TaDrAp2 and TaRht-B1 genes. Alleles of two particular homeologs, TaDrAp1-B4 and TaDrAp2-B1, co-segregated with grain yield in nine selected breeding lines. This indicated an important regulatory role for both TaDrAp1 and TaDrAp2 genes in plant growth, ontogenesis, and drought tolerance in bread and spelt wheat.This publication has 77 references indexed in Scilit:
- HVP10 encoding V-PPase is a prime candidate for the barley HvNax3 sodium exclusion gene: evidence from fine mapping and expression analysisPlanta, 2013
- Genus Triticum L. taxonomy: the present and the futureÖsterreichische botanische Zeitschrift, 2011
- Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyonPLOS ONE, 2011
- Genome sequencing and analysis of the model grass Brachypodium distachyonNature, 2010
- The Yeast RNA Polymerase II-associated Factor Iwr1p Is Involved in the Basal and Regulated Transcription of Specific GenesOnline Journal of Public Health Informatics, 2009
- Identification and validation of reference genes for quantitative RT-PCR normalization in wheatBMC Molecular Biology, 2009
- Identification, characterization and interaction of HAP family genes in riceMolecular Genetics and Genomics, 2008
- Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acresProceedings of the National Academy of Sciences of the United States of America, 2007
- Application of Phylogenetic Networks in Evolutionary StudiesMolecular Biology and Evolution, 2005
- Basic local alignment search toolJournal of Molecular Biology, 1990