Apoptosis correction in nephrotic children

Abstract

Hypoxia-related disorders play an important role in irreversible kidney tissue damage via activation of certain secondary processes, i.e. apoptosis. This study aimed to investigate the levels of apoptosis controlling factors and nuclear transcriptional factor NF-κB in relation to the value of kidney function impairment; to evaluate possible protective effects of conventional therapy with the addition of an antioxidant agent tocopherol in pediatric patients with nephrotic syndrome. Methods. An examination of blood samples of 53 patients (aged 10 to 15 years) with nephrotic syndrome was performed. Conventional clinical investigations, immunoblotting were used in this study. Results. We demonstrate that nephrotic patients reveal a high level of pro-apoptotic marker Bax, low level of anti-apoptotic factor BcL-xL and anti-apoptotic unit of NF-κB (p65). Their dependence on level of kidney function impairment was detected. Applied treatment composes conventional scheme and tocopherol shows a restored balance of apoptosis controlling factor and NF-κB activity. Conclusion. Hypoxia-induces disorders in nephrotic children resulted in apoptosis controlling system disturbances can be attenuated by the antioxidants application.